Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Department of Biology, Science and Research Branch Islamic Azad University, Tehran, Iran.
Can J Gastroenterol Hepatol. 2022 Nov 8;2022:2045572. doi: 10.1155/2022/2045572. eCollection 2022.
IBD is an inflammatory disease with abnormalities such as dysbiosis and abnormal immune system activity. Probiotics, as live beneficial microorganisms, play a role in maintaining health through various mechanisms, including the modulation of the immune system and the control of inflammation. Here, we aimed to investigate the efficacy of a probiotic mixture of spp. and spp. in modulating JAK/STAT and NF-kB inflammatory signaling pathways.
A quantitative real-time polymerase chain reaction (qPCR) assay was conducted to analyze the expression of JAK/STAT and inflammatory genes after treatment with the probiotic mixture before, after, and simultaneously with the sonicated pathogen in the HT-29 cell line. The production of IL-6 and IL-1 after probiotic treatment was investigated via cytokine assay.
Treatment with probiotics resulted in downregulation of , , , and genes in the NF-kB pathway and / genes compared with sonicat-treated cells as inflammation inducers. The production of IL-6 and IL-1 decreased after probiotic treatment.
The probiotic mixture of spp. and spp. showed anti-inflammatory effects by modulating JAK/STAT and NF-kB signaling pathways. The use of probiotics could be considered as an appropriate complementary treatment for patients with inflammatory bowel disease.
IBD 是一种炎症性疾病，存在着微生物群落失调和免疫系统异常等异常情况。益生菌作为有益的活体微生物，通过调节免疫系统和控制炎症等多种机制，在维持健康方面发挥作用。在这里，我们旨在研究 spp. 和 spp. 益生菌混合物在调节 JAK/STAT 和 NF-kB 炎症信号通路方面的功效。
通过定量实时聚合酶链反应 (qPCR) 分析，在 HT-29 细胞系中，在用 sonicat 处理的病原体之前、之后和同时用益生菌混合物处理后，分析 JAK/STAT 和炎症基因的表达。通过细胞因子分析研究益生菌处理后 IL-6 和 IL-1 的产生情况。
与作为炎症诱导剂的 sonicat 处理细胞相比，益生菌处理导致 NF-kB 通路中的 和 基因以及 / 基因下调。益生菌处理后 IL-6 和 IL-1 的产生减少。
spp. 和 spp. 的益生菌混合物通过调节 JAK/STAT 和 NF-kB 信号通路显示出抗炎作用。益生菌的使用可以被认为是炎症性肠病患者的一种合适的补充治疗方法。
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