Sjöstrand F S, Candipan R C
J Ultrastruct Res. 1985 Apr;91(1):38-50. doi: 10.1016/0889-1605(85)90074-6.
Contrary to the generally accepted rule that there are only two fracture faces associated with a membrane, the analysis of double replicas at rat heart muscle mitochondria revealed three pairs of complementary replicas with one face in each pair exposing the outer surface membrane. The replicas must then expose the surfaces of the outer surface membrane and in two of the pairs the fracture had passed between the two surface membranes in two alternative ways, either clearly between the two membranes or the fracture deviated into and through the inner surface membrane at regularly spaced intervals. This deviation reveals that at these sites the connection between the two surface membranes is particularly firm. The analysis led to the conclusion that these sites correspond to those where the stalk-like connections extending from the cristae are connected to the inner surface membrane. This way proteinaceous pathways connect the cristae to the surface of the mitochondria.
与普遍接受的规则（即与膜相关的只有两个断裂面）相反，对大鼠心肌线粒体双复制品的分析显示有三对互补复制品，每对中的一个面暴露外表面膜。这些复制品必定暴露了外表面膜的表面，并且在其中两对中，断裂以两种交替方式穿过两个表面膜之间，要么明显在两个膜之间，要么断裂以规则间隔偏向并穿过内表面膜。这种偏向表明在这些位点两个表面膜之间的连接特别牢固。该分析得出的结论是，这些位点对应于从嵴延伸出的茎状连接与内表面膜相连的地方。通过这种方式，蛋白质通道将嵴与线粒体表面连接起来。
