Rayapati Ananda Murali, Chandrasekhar Chanda, Poojari Sudarsana, Vemulapati Bhadra Murthy
Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur 522502, India.
Cool Climate Oenology and Viticulture Institute, Brock University, St. Catharines, ON L2S 3A1, Canada.
Viruses. 2025 Jun 7;17(6):827. doi: 10.3390/v17060827.
Myricetin, a flavonoid compound, was demonstrated to effectively arrest the cell-to-cell fusion and syncytial development triggered by Nipah virus (NiV) fusion (F) and attachment (G) envelope glycoproteins in vitro involving two permissive mammalian cell lines. Time-of-addition assays were carried out using codon-optimized NiV wild type (WT) F and G plasmids followed by a challenge with the addition of myricetin 1 h and 6 h post-transfection in HEK 293T and Vero cells. Upon evaluating different myricetin concentrations, it was determined that a 100 μM concentration of myricetin effectively inhibited 64-80% of syncytia in HEK and Vero cells. In this study, we concluded that myricetin mitigated the syncytial development in HEK and Vero cell lines. Given the flavonoid attributes of myricetin which is widely present in fruits, vegetables, tea, and wine, it may be regarded as a phytonutrient and a safer antiviral alternative against Nipah virus infections. Due to the BSL-4 nature of the virus, further research involving live virus culture is necessary to confirm myricetin as a potential antiviral compound for the mitigation of pathological effects of NiV infections.
杨梅素是一种黄酮类化合物，已证明它能在体外有效阻止由尼帕病毒（NiV）融合（F）和附着（G）包膜糖蛋白引发的细胞间融合和多核体发育，该实验涉及两种易感哺乳动物细胞系。使用密码子优化的NiV野生型（WT）F和G质粒进行添加时间试验，随后在转染后1小时和6小时向HEK 293T和Vero细胞中添加杨梅素进行挑战。在评估不同浓度的杨梅素后，确定100μM浓度的杨梅素能有效抑制HEK和Vero细胞中64 - 80%的多核体。在本研究中，我们得出结论，杨梅素可减轻HEK和Vero细胞系中的多核体发育。鉴于杨梅素的黄酮类属性广泛存在于水果、蔬菜、茶和葡萄酒中，它可被视为一种植物营养素和对抗尼帕病毒感染的更安全的抗病毒替代品。由于该病毒具有生物安全等级4的特性，有必要进行涉及活病毒培养的进一步研究，以确认杨梅素作为一种潜在的抗病毒化合物，用于减轻NiV感染的病理影响。
