Vita Dikudila G, Santiso-Bellón Cristina, Lemos Manuel, Neto Zoraima, Fortes-Gabriel Elsa, Brito Miguel, Sebastião Cruz S, Rodriguez-Diaz Jesus, Cunha Celso, Istrate Claudia
Faculty of Medicine, Agostinho Neto University, Luanda P.O. Box 116, Angola.
Department of Microbiology, School of Medicine, University of Valencia, Av. Blasco Ibáñez 15, 46010 Valencia, Spain.
Viruses. 2025 Jun 17;17(6):858. doi: 10.3390/v17060858.
(. ) remains the leading cause of pediatric diarrhea. Although Angola introduced Rotarix, the human monovalent . vaccine since 2014 as part of its routine childhood immunization program, no follow-up study has been conducted. The aim of this study was to evaluate the distribution of genotypes among children under five years of age, hospitalized with acute gastroenteritis (AGE), after the introduction of the rotavirus vaccine. To achieve this goal, stool samples collected between 2021 and 2022 from children under 5 years of age diagnosed with AGE at six hospitals in Luanda Province were analyzed. The -antigen immunochromatographic test (SD Bioline™, Abbott, Chicago, IL, USA) was performed, and 121 positive samples were genotyped. Ten samples were randomly selected for further Sanger sequencing. The results showed that the G9P[6] was the most prevalent genotype (17.3%), followed by G9P[8] (16.5%), G2P[4] (14.9%), G3P[6] (13.2%), G8P[6] (11.5%), and less frequently G12P[8] (9.1%), G1P[6] (4.1%), and G1P[8] (2.5%). The genotype combinations G3P[6], G8P[6], and G12P[8] were detected for the first time in Luanda Province. In conclusion, the emergence of new genotype combinations supports the need for continuous surveillance to identify the trend in infection and the emergence of new strains circulating in Luanda Province in the post-vaccination period.
（. ）仍然是小儿腹泻的主要原因。尽管安哥拉自2014年起将人单价. 疫苗Rotarix作为其儿童常规免疫规划的一部分引入，但尚未进行后续研究。本研究的目的是评估轮状病毒疫苗引入后，五岁以下因急性胃肠炎（AGE）住院儿童中 基因型的分布情况。为实现这一目标，对2021年至2022年期间从罗安达省六家医院诊断为AGE的五岁以下儿童收集的粪便样本进行了分析。进行了 -抗原免疫层析试验（SD Bioline™，雅培公司，美国伊利诺伊州芝加哥），并对121份阳性样本进行了基因分型。随机选择10份样本进行进一步的桑格测序。结果显示，G9P[6]是最常见的基因型（17.3%），其次是G9P[8]（16.5%）、G2P[4]（14.9%）、G3P[6]（13.2%）、G8P[6]（11.5%），较少见的是G12P[8]（9.1%）、G1P[6]（4.1%）和G1P[8]（2.5%）。基因型组合G3P[6]、G8P[6]和G12P[8]在罗安达省首次被检测到。总之，新基因型组合的出现支持了在疫苗接种后时期持续监测的必要性，以确定罗安达省 感染的趋势和新毒株的出现情况。
