Montenegro Iván, Fuentes Bastián, Silva Valentina, Valdés Francisca, Werner Enrique, Santander Rocío, Moraga-Espinoza Daniel, Madrid Alejandro
Center of Interdisciplinary Biomedical and Engineering Research for Health (MEDING), Escuela de Obstetricia y Puericultura, Facultad de Medicina, Universidad de Valparaíso, Angamos 655, Reñaca, Viña del Mar 2520000, Chile.
Laboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, Chile.
Pharmaceutics. 2025 Jun 8;17(6):755. doi: 10.3390/pharmaceutics17060755.
Candidiasis, an opportunistic fungal infection caused by Candida species, is a major health problem, particularly in immunocompromised individuals. Increasing resistance of yeasts such as spp. to pharmacological antifungal agents makes it necessary to explore alternative treatments. The aim of this study was to evaluate the antifungal potential of essential oil (GKEO) against spp. by assessing growth and development at 24 and 48 h. Encapsulation and characterization of a stable nanoemulsion were carried out to enhance efficacy. : The anti-yeast activity of both free GKEO and the nanoemulsion against , , and was evaluated using a microdilution method to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) at 24 and 48 h. : GC-MS/MS analysis identified forty-one components in GKEO, the main ones being eucalyptol (21.41%), 4-terpineol (19.62%), and α-terpinyl acetate (13.89%). Antifungal assays revealed that both free and nanoemulsion-formulated GKEO inhibited the growth of all tested strains. At 48 h, the nanoformulated GKEO achieved a MIC value of 32 µg/mL and an MFC of 64 µg/mL for and and showed higher antifungal activity compared to the free oil, in particular against , exhibiting a four-fold lower MFC value. The activity of the nanoformulation was comparable to or better than fluconazole against and . : The GKEO nanoemulsion potentiated anti-yeast activity against spp. The formulation improved the efficacy of GKEO, suggesting its potential as an alternative or adjunctive treatment for candidiasis.
念珠菌病是由念珠菌属引起的一种机会性真菌感染，是一个主要的健康问题，在免疫功能低下的个体中尤为如此。诸如某些念珠菌属等酵母菌对药理学抗真菌剂的耐药性不断增加，使得有必要探索替代治疗方法。本研究的目的是通过评估24小时和48小时时的生长和发育情况，来评价[某种植物]精油（GKEO）对某些念珠菌属的抗真菌潜力。进行了稳定纳米乳液的包封和表征以提高疗效。：使用微量稀释法评估游离GKEO和纳米乳液对[几种念珠菌]的抗酵母菌活性，以确定24小时和48小时时的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。：气相色谱 - 质谱/质谱分析确定了GKEO中的41种成分，主要成分是桉叶油素（21.41%）、4 - 萜品醇（19.62%）和乙酸α - 萜品酯（13.89%）。抗真菌试验表明，游离和纳米乳液形式的GKEO均抑制了所有测试的念珠菌菌株的生长。在48小时时，纳米配方的GKEO对[某些念珠菌]的MIC值为32μg/mL，MFC为64μg/mL，并且与游离油相比显示出更高的抗真菌活性，特别是对[某种念珠菌]，其MFC值低四倍。纳米制剂的活性与氟康唑对[某些念珠菌]的活性相当或更好。：GKEO纳米乳液增强了对某些念珠菌属的抗酵母菌活性。该制剂提高了GKEO的疗效，表明其作为念珠菌病替代或辅助治疗的潜力。
