Xu Liugen, Pi Junwei, Feng Lei, Wen Junhao, Zhao Minghai, Ali Amjad, Lu Jianwei, Guo Li
School of Materials Sciences and Engineering, Jiangsu University, Zhenjiang 212013, China.
College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China.
Polymers (Basel). 2025 Jun 8;17(12):1600. doi: 10.3390/polym17121600.
Glycopolypeptoids were synthesized and showed effective antifreeze activity, demonstrating their potential as novel antifreeze agents for cryopreservation. These polypeptide analogs offer improved stability and tunability compared with natural antifreeze glycoproteins (AFGPs) and existing synthetic mimics. Using the ring-opening polymerization of N-substituted N-carboxyanhydride monomers followed by click chemistry, glycopolypeptoids with controlled polymerization degrees and varied structures were designed and prepared. Their antifreeze performance was assessed via nanoliter osmometry and ice recrystallization inhibition assays, revealing a strong correlation between the molecular structure and antifreeze activity. The findings highlight glycopolypeptoids as a promising, cost-effective alternative to natural AFGPs, providing new insights into the development of biomimetic cryoprotectants. This study expands the understanding of synthetic antifreeze materials and offers a practical approach for improving cryopreservation efficiency in biomedical and industrial applications.
合成了糖多肽类化合物，其表现出有效的抗冻活性，证明了它们作为新型冷冻保护抗冻剂的潜力。与天然抗冻糖蛋白（AFGP）和现有的合成模拟物相比，这些多肽类似物具有更高的稳定性和可调节性。通过N-取代的N-羧基酸酐单体的开环聚合反应，随后进行点击化学，设计并制备了具有可控聚合度和不同结构的糖多肽类化合物。通过纳升渗透压测定法和冰重结晶抑制试验评估了它们的抗冻性能，揭示了分子结构与抗冻活性之间的强相关性。这些发现突出了糖多肽类化合物作为天然AFGP的一种有前景、经济高效的替代品，为仿生冷冻保护剂的开发提供了新的见解。这项研究扩展了对合成抗冻材料的理解，并为提高生物医学和工业应用中的冷冻保存效率提供了一种实用方法。
