Zhao Huaxuan, Zhang Guichao, Liu Jiarong, Mo Futian, Li Taoen, Lei Chengyong, Lü Shidong
School of Stomatology, Southern Medical University, Guangzhou 510515, China.
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2025 Jun 20;45(6):1280-1288. doi: 10.12122/j.issn.1673-4254.2025.06.17.
To investigate the characteristics of immune cell infiltration in tumor samples from Chinese patients with clear cell renal cell carcinoma (ccRCC) and the correlation of immune cell infiltration with tumor stage and response to immunotherapy.
Tumor samples and clinicopathological data were collected from 154 ccRCC patients treated in Nanfang Hospital, Southern Medical University from October, 2020 to October, 2023. The immune cell types infiltrating the tumor tissues were identified using immunohistochemistry and immunofluorescence staining, and their correlations with the patients' clinicopathological characteristics were analyzed. Patient-derived tumor tissue fragment models (PDTF) models, constructed using tumor tissues from 22 patients, were treated with PD-1 monoclonal antibody, and T cell activation was detected using flow cytometry to assess the patients' responses to immunotherapy.
In Chinese ccRCC patients included in this study, CD8 T cells, CD4 T cells, and CD3 T cells were the most abundant in the tumor tissues. Higher infiltration levels of CD3 T cells (=0.004), PD-1 T cells (=0.020), CD68 T cells (=0.049), CD79 T cells (=0.049), and Tryptase cells (=0.049) were all positively correlated with a larger tumor size (≥5 cm). A higher infiltration level of CD4 T cells was associated with a lower tumor stage. Patients with higher International Society of Urological Pathology (ISUP) grades had higher infiltration levels of CD3 T cells (=0.023), CD8 T cells (=0.045), PD-1 T cells (=0.014), CD20 B cells (=0.020) and CD79 B cells (=0.049), and lower levels of Tryptase cells (=0.001). Patients with abundant infiltrating immune cells tended to have better responses to immunotherapy.
The infiltrating immune cells are heterogeneous in Chinese ccRCC patients, and immune cell infiltration characteristics are closely correlated with clinicopathological parameters of the patients.
探讨中国透明细胞肾细胞癌（ccRCC）患者肿瘤样本中免疫细胞浸润的特征，以及免疫细胞浸润与肿瘤分期和免疫治疗反应的相关性。
收集2020年10月至2023年10月在南方医科大学南方医院接受治疗的154例ccRCC患者的肿瘤样本和临床病理数据。采用免疫组织化学和免疫荧光染色鉴定浸润肿瘤组织的免疫细胞类型，并分析其与患者临床病理特征的相关性。使用22例患者的肿瘤组织构建患者来源的肿瘤组织片段模型（PDTF），用PD-1单克隆抗体处理，通过流式细胞术检测T细胞活化，以评估患者对免疫治疗的反应。
在本研究纳入的中国ccRCC患者中，肿瘤组织中CD8 T细胞、CD4 T细胞和CD3 T细胞最为丰富。CD3 T细胞（=0.004）、PD-1 T细胞（=0.020）、CD68 T细胞（=0.049）、CD79 T细胞（=0.049）和类胰蛋白酶细胞（=0.049）浸润水平较高均与较大肿瘤大小（≥5 cm）呈正相关。CD4 T细胞浸润水平较高与较低肿瘤分期相关。国际泌尿病理学会（ISUP）分级较高的患者CD3 T细胞（=0.023）、CD8 T细胞（=0.045）、PD-1 T细胞（=0.014）、CD20 B细胞（=0.020）和CD79 B细胞（=0.049）浸润水平较高，类胰蛋白酶细胞水平较低（=0.001）。浸润免疫细胞丰富的患者对免疫治疗的反应往往更好。
中国ccRCC患者浸润免疫细胞具有异质性，免疫细胞浸润特征与患者临床病理参数密切相关。
