Activity of lactate dehydrogenase, acid and alkaline phosphatases in measles virus-infected monkey kidney cell cultures.

The influence of wild (Co-69) and attenuated (L-16) measles virus infection on the activity and activation energy of lactate dehydrogenase (LDH), acid and alkaline phosphatases (P-ase) was tested in R9CA monkey kidney cells at various time intervals after inoculation. With both virus strains significant changes in enzymatic activity occurred especially within the first 24 hours post inoculation. The two stains have a different influence on alkaline P-ase activity variation, which might therefore be used as a differentiation marker. Variations in LDH activation energy might be an early indicator of virus infection. LDH activity is significantly higher in cellular homogenates diluted 1:10, the dilution effect being more obvious in infected cells after a longer time interval post inoculation.