Harjula A, Mattila S, Myllärniemi H, Mattila I, Mattila P, Salmenperä M, Merikallio E
J Surg Res. 1985 Nov;39(5):405-12. doi: 10.1016/0022-4804(85)90094-0.
In an experimental study the effects of Fluosol DA (added with potassium chloride) on the vascular interface and endothelial cells were compared to those of crystalloid potassium cardioplegic solution using scanning electron microscope. Twenty rabbits (10 in each group) were sacrificed, the hearts with ascending aorta were immediately excised, and cold oxygenated solution was infused via a cannula inserted into the cross-clamped aorta. The hearts were left immersed in the perfusion medium for 2 hr. In the Fluosol DA group endothelial cover and endothelial cells were normal or minimal changes were seen in seven cases. Occasional breaking of intercellular attachments, small areas of denuded flow surface, and disappearance of microvilli were seen in three cases. In the crystalloid potassium cardioplegic group 7 of the 10 cases showed moderate or severe damage with large areas of denuded flow surface. The present experimental protocol represented an extreme situation where no collateral coronary blood was present. The coronary endothelial damage was obvious after the crystalloid potassium cardioplegic solution. Similar damage was not found following Fluosol DA infusion.
在一项实验研究中，使用扫描电子显微镜将氟碳乳剂DA（添加氯化钾）对血管界面和内皮细胞的影响与晶体钾停搏液的影响进行了比较。处死20只兔子（每组10只），立即切除带有升主动脉的心脏，并通过插入夹闭主动脉的插管注入冷氧合溶液。将心脏浸泡在灌注介质中2小时。在氟碳乳剂DA组中，7例内皮覆盖和内皮细胞正常或仅有微小变化。3例可见细胞间连接偶尔断裂、小面积的裸露血流表面和微绒毛消失。在晶体钾停搏液组中，10例中有7例显示中度或重度损伤，有大面积的裸露血流表面。本实验方案代表了一种极端情况，即不存在侧支冠状动脉血流。晶体钾停搏液灌注后冠状动脉内皮损伤明显。氟碳乳剂DA灌注后未发现类似损伤。
