Manjunatha C, Aditya K, Prasannakumar M K, Pramesh D, Buella P, Patil Swathi S, Venkateshbabu Gopal, Harish J, Karan R, Ramegowda H V
Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, Bengaluru, India.
PathoGenOmics Lab, Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
Methods Mol Biol. 2025;2943:47-64. doi: 10.1007/978-1-0716-4642-7_4.
This chapter reviews various isothermal amplification techniques, which are alternative to PCR for detecting plant pathogens. These methods, including NASBA, SDA, LAMP, HDA, and RPA, amplify nucleic acids at a constant temperature, making them potentially more suitable for point-of-care (POC) applications. The review compares these techniques regarding their mechanisms, advantages, and limitations, highlighting their potential for rapid, cost-effective, and on-site plant disease diagnosis, including the integration of these techniques with CRISPR-Cas systems and lab-on-a-chip technologies.
本章回顾了各种等温扩增技术，这些技术是用于检测植物病原体的聚合酶链式反应（PCR）的替代方法。这些方法，包括核酸序列扩增技术（NASBA）、链置换扩增技术（SDA）、环介导等温扩增技术（LAMP）、螺旋酶依赖性扩增技术（HDA）和重组酶聚合酶扩增技术（RPA），可在恒温下扩增核酸，使其可能更适合即时检测（POC）应用。本综述比较了这些技术在机制、优点和局限性方面的情况，强调了它们在快速、经济高效的现场植物病害诊断方面的潜力，包括这些技术与CRISPR-Cas系统和芯片实验室技术的整合。
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