Rashid J S, Rahman P, Khan R R, Afrin S S, Mudi N, Saha M K, Yeasmin F, Karim S S, Dewan R K, Zaman P, Rahman M
Dr Jubyda Shahnur Rashid, Assistant Professor, Department of Pathology, Sir Salimullah Medical College, Dhaka, Bangladesh.; E-mail:
Mymensingh Med J. 2025 Jul;34(3):816-824.
Nowadays, human papilloma virus (HPV) testing has been added with cytology to aid in detection of high-grade cervical lesions, especially in women aged ≥30 years. This study aimed to evaluate the relationship between high-risk HPV (hrHPV) genotype and clinicopathological parameters among the diagnosed patients with both precancerous and cancerous cervical lesions. This cross-sectional study was carried out in the Department of Pathology of Dhaka Medical College and in the collaboration with 'DNA Solution LTD, Dhaka', Bangladesh. The study was conducted over a period of 24 months from July 2018 to June 2020. Following informed written consent, a total of 51 women (18 patients with clinically suspected malignant lesions and 33 with a positive VIA test) were included in this study, and all were subjected to colposcopic examination performed by a gynecologist. Ethical issues were ensured in accordance with the 1975 Declaration of Helsinki. SPSS version 24.0 was used during data analysis. The average age ±SD of the patients with cervical lesions was 41.86±9.84 years with a range from 25 to 60 years. Approximately half of the participants (n=25, 49.0%) were married for less than 16 years and almost one-tenth were multiparous. Among all cytological and histological findings, hrHPV-16 (n=21, 41.18%) was commonest, followed by hrHPV-18 (n=3, 5.88%) and hrHPV-68 (n=1, 1.96%). Two patients had co-infection with hrHPV (16, 18) and hrHPV (16, 51) in particular. However, the rest 24 cases remained undetected. Among the hrHPV-positive cases (n=27), hrHPV-16 was detected in 5 cases while those were negative for intraepithelial lesions. In cervical swabs, 6 cases of hrHPV-16, 2 cases of hrHPV-18 and one case of co-infection hrHPV (16, 18) were histologically diagnosed as invasive SCC G-II. Among 51 cases, the most common histological diagnosis was CIN I (n=18, 35.3%). hrHPV genotyping was found to be associated with cytological findings and histological diagnosis.
如今，人乳头瘤病毒（HPV）检测已与细胞学检查相结合，以帮助检测高级别宫颈病变，尤其是在30岁及以上的女性中。本研究旨在评估诊断为宫颈癌前病变和癌性病变患者中高危型HPV（hrHPV）基因型与临床病理参数之间的关系。这项横断面研究在达卡医学院病理学系进行，并与孟加拉国达卡的“DNA Solution LTD”合作开展。该研究从2018年7月至2020年6月，历时24个月。在获得知情书面同意后，本研究共纳入51名女性（18例临床疑似恶性病变患者和33例醋酸白试验阳性患者），所有患者均接受了妇科医生进行的阴道镜检查。按照1975年《赫尔辛基宣言》确保了伦理问题。数据分析使用SPSS 24.0版。宫颈病变患者的平均年龄±标准差为41.86±9.84岁，年龄范围为25至60岁。大约一半的参与者（n = 25，49.0%）结婚不到16年，近十分之一为经产妇。在所有细胞学和组织学检查结果中，hrHPV - 16（n = 21，41.18%）最为常见，其次是hrHPV - 18（n = 3，5.88%）和hrHPV - 68（n = 1，1.96%）。特别是有2例患者同时感染了hrHPV（16，18）和hrHPV（16，51）。然而，其余24例未检测到。在hrHPV阳性病例（n = 27）中，有5例检测到hrHPV - 16，而这些病例的上皮内病变为阴性。在宫颈拭子中，6例hrHPV - 16、2例hrHPV - 18和1例hrHPV（16，18）合并感染在组织学上被诊断为浸润性鳞状细胞癌G-II级。在51例病例中，最常见的组织学诊断为CIN I（n = 18，35.3%）。发现hrHPV基因分型与细胞学检查结果和组织学诊断相关。
