Du Dan, Gong Xiao-Wei, Yuan Ya-Dong
Department of Respiratory and Critical Care Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, China.
Iran J Basic Med Sci. 2025;28(8):1019-1026. doi: 10.22038/ijbms.2025.82437.17822.
Pulmonary arterial hypertension (PAH) is a severe heart-lung condition characterized by complex changes in the pulmonary vasculature, known as pulmonary vascular remodeling (PVR). Gypenosides (Gyp) possesses a range of pharmacological properties, including anti-inflammatory and anti-oxidant effects. This study aims to explore the impact of Gyp on pulmonary vascular remodeling, particularly in relation to its potential to counteract inflammation, oxidative stress, and apoptosis.
Twenty-four rats were randomly divided into four groups. MCT was administered via intraperitoneal injection at a 55 mg/kg dose to establish a PAH model. Gyp (150 mg/kg/day, Ig) was administered for 28 days, after which all lung tissues from the rats were isolated.
The findings indicated that Gyp had a substantial positive impact on the hemodynamics of rats with PAH induced by MCT, including reductions in mean pulmonary artery pressure (mPAP) and right ventricular systolic pressure (RVSP). Additionally, it exhibited inhibitory effects on right ventricular hypertrophy and pulmonary vascular remodeling in these PAH-afflicted rats. MCT elevated the concentration of MDA (<0.01 in the lung) while reducing the levels of SOD and GSH-PX (<0.0001). Furthermore, MCT enhanced the expression of IL-6, TNF-α, and IL-1β (<0.0001), as well as the mRNA expression of NF-κB (<0.001) and the Bcl2 level (<0.0001), while it lowered the expression of Bax (<0.0001). Conversely, Gyp treatment effectively mitigated all of these alterations.
This study represents the initial investigation showing that Gyp treatment attenuates PVR by inhibiting oxidative stress, inflammation, and apoptosis, providing a foundation for further research.
肺动脉高压（PAH）是一种严重的心肺疾病，其特征是肺血管发生复杂变化，即肺血管重塑（PVR）。绞股蓝总苷（Gyp）具有一系列药理特性，包括抗炎和抗氧化作用。本研究旨在探讨Gyp对肺血管重塑的影响，特别是其对抗炎症、氧化应激和细胞凋亡的潜力。
24只大鼠随机分为四组。通过腹腔注射55mg/kg剂量的野百合碱（MCT）建立PAH模型。给予Gyp（150mg/kg/天，灌胃）28天，之后分离所有大鼠的肺组织。
研究结果表明，Gyp对MCT诱导的PAH大鼠的血流动力学有显著的积极影响，包括降低平均肺动脉压（mPAP）和右心室收缩压（RVSP）。此外，它对这些PAH患病大鼠的右心室肥大和肺血管重塑具有抑制作用。MCT升高了丙二醛（MDA）的浓度（肺中<0.01），同时降低了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）的水平（<0.0001）。此外，MCT增强了白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的表达（<0.0001），以及核因子-κB（NF-κB）的mRNA表达（<0.001）和Bcl2水平（<0.0001），同时降低了Bax的表达（<0.0001）。相反，Gyp治疗有效地减轻了所有这些改变。
本研究是首次表明Gyp治疗通过抑制氧化应激、炎症和细胞凋亡来减轻PVR的研究，为进一步研究奠定了基础。
