Abrantes Pedro Miguel Dos Santos, Chesnay Stuurman Keith, Arthur Klaasen Jeremy, Joyce Africa Charlene Wilma
Maternal Endogenous Infections Studies (MEnIS) Research Laboratories, Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa.
Plant Extract Laboratory, Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa.
Curr Med Mycol. 2024 Nov 16;10. doi: 10.22034/cmm.2024.345240.1541. eCollection 2024.
Yeasts of the genus are responsible for localized and disseminated infections, especially in immunocompromised populations. These infections are exacerbated by the rapid increase in drug-resistant strains, which limits treatment options and increases patient morbidity and mortality. Therefore, the utilization of easily accessible natural products as alternatives to conventional medicines has gained interest. South Africa is home to a rich biodiverse natural flora of which many are known for their antimicrobial activity, including the antifungal effects of their plant extracts. (kraalbos) is a local indigenous plant found to have various traditional uses, including the treatment and prevention of various human infections.
In this study, the activity of against and preformed biofilm formation and its antibiofilm activity were tested using the xCELLigence system, which monitors biofilm formation in real time using impedance.
Presence of resulted in a dose-dependent decrease in biofilms and was found to be effective in the prevention of biofilm formation and disruption of the existing biofilms.
The xCELLigence impedance-based system proved to be an effective tool for medication screening. To the best of our knowledge, this is the first reported study to use real-time monitoring of a medicinal plant on microbial biofilm formation.
该属酵母菌可引发局部和播散性感染，尤其是在免疫功能低下人群中。耐药菌株的迅速增加加剧了这些感染，这限制了治疗选择并增加了患者的发病率和死亡率。因此，利用易于获取的天然产物作为传统药物的替代品受到了关注。南非拥有丰富多样的天然植物群，其中许多植物以其抗菌活性而闻名，包括其植物提取物的抗真菌作用。（ kraalbos）是一种当地本土植物，被发现有多种传统用途，包括治疗和预防各种人类感染。
在本研究中，使用xCELLigence系统测试了 对 以及预先形成的生物膜形成及其抗生物膜活性，该系统使用阻抗实时监测生物膜形成。
的存在导致 生物膜呈剂量依赖性减少，并且被发现可有效预防 生物膜形成并破坏现有的 生物膜。
基于xCELLigence阻抗的系统被证明是药物筛选的有效工具。据我们所知，这是首次报道使用药用植物对微生物生物膜形成进行实时监测的研究。
