Alqarni Saad, Zaki Magdi E A, Alruwaili Awatif H, El-Naggar Mohamed, Alafnan Ahmed, Almansour Khaled, Huwaimel Bader, Abouzied Amr S, Gomha Sobhi M
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Ha'il, Hail, Saudi Arabia.
Department of Chemistry, Faculty of Science, Imam Mohammed Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia.
J Biochem Mol Toxicol. 2025 Jul;39(7):e70367. doi: 10.1002/jbt.70367.
This study reports the design, synthesis, and antimicrobial evaluation of novel thiazole-based benzylidene thiazolidinones. Starting from 4-thiazolidinone intermediates (3a and 3b), benzylidene derivatives (5a-l, 7a,b) and bis-derivatives (9a,b) were synthesized via condensation with aromatic aldehydes. In vitro antimicrobial screening against Gram-positive and Gram-negative bacteria, yeast, and fungi showed several compounds-especially 5 f and 5 h-exhibited significant activity comparable to gentamicin and fluconazole. Molecular docking against the Staphylococcus aureus gyrase-DNA complex further supported the experimental results, revealing strong binding affinities for select compounds, particularly 9b. These findings suggest the synthesized thiazolidinones are promising candidates for developing new antimicrobial agents targeting resistant pathogens.
本研究报告了新型噻唑基亚苄基噻唑烷酮的设计、合成及抗菌活性评估。以4-噻唑烷酮中间体（3a和3b）为起始原料，通过与芳香醛缩合合成了亚苄基衍生物（5a-l、7a,b）和双衍生物（9a,b）。针对革兰氏阳性菌、革兰氏阴性菌、酵母和真菌的体外抗菌筛选表明，几种化合物——尤其是5f和5h——表现出与庆大霉素和氟康唑相当的显著活性。针对金黄色葡萄球菌促旋酶-DNA复合物的分子对接进一步支持了实验结果，揭示了所选化合物，特别是9b，具有很强的结合亲和力。这些发现表明，合成的噻唑烷酮有望成为开发针对耐药病原体的新型抗菌剂的候选药物。
