Szabó G, Kovács G L, Penke B, Telegdy G
Neuropeptides. 1985 Sep;6(5):397-404. doi: 10.1016/0143-4179(85)90138-6.
The effects of graded doses of cholecystokinin octapeptide sulphate (CCK-8-S) and non-sulphated CCK-8 (CCK-8-NS) on the development of tolerance to the hypothermic effect of ethanol were investigated in mice. The animals were pretreated with daily doses of CCK-8-S, CCK-8-NS (0.003-30 nmol/animal s.c.) or vehicle, followed two hours later by the i.p. administration of ethanol (4 g/kg). The same treatments were repeated on three consecutive days. The hypothermic response to ethanol administration gradually diminished, this phenomenon being accepted as indicating the development of tolerance to ethanol. Both CCK-8-S and CCK-8-NS inhibited the development of tolerance. While CCK-8-S resulted in a linear dose-response effect (0.3-30 nmol/animal), CCK-8-NS gave rise to a bell-shaped curve with effective blocking doses of 0.3-3 nmol/animal. There was no difference in colonic temperature between peptide and vehicle-treated, ethanol-naive animals, indicating that the peptide did not change the initial sensitivity to ethanol.
研究了不同剂量的硫酸胆囊收缩素八肽（CCK-8-S）和非硫酸化CCK-8（CCK-8-NS）对小鼠乙醇低温效应耐受性发展的影响。动物每天皮下注射CCK-8-S、CCK-8-NS（0.003 - 30 nmol/只）或溶剂，两小时后腹腔注射乙醇（4 g/kg）。连续三天重复相同处理。乙醇给药后的低温反应逐渐减弱，这种现象被认为表明对乙醇耐受性的发展。CCK-8-S和CCK-8-NS均抑制耐受性的发展。虽然CCK-8-S产生线性剂量反应效应（0.3 - 30 nmol/只），但CCK-8-NS产生钟形曲线，有效阻断剂量为0.3 - 3 nmol/只。肽处理组和溶剂处理组、未接触过乙醇的动物结肠温度无差异，表明肽未改变对乙醇的初始敏感性。
