Farooq Asmat, Sharma Vikas, Sofi Sajad Ahmad, Bashir Saika, Rai Pradeep Kumar, Sofi Parvaze Ahmad, Deveshwar Priyanka, Zargar Sajad Majeed
Natural Product Laboratory, Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Chatha 180009, India; Proteomics Laboratory, Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar 190025, India.
Natural Product Laboratory, Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Chatha 180009, India.
Plant Sci. 2025 Oct;359:112632. doi: 10.1016/j.plantsci.2025.112632. Epub 2025 Jun 28.
Common bean (Phaseolus vulgaris) is one of the most important legume crops for human consumption because of its nearly perfect nutritional composition. However, the crop yield is invariably constrained by phosphorus (P) stress across all farming systems. The present study aimed to unravel the mechanistic basis of common bean response to P stress at the early vegetative phase using an integrated multi-omic approach. In vitro experiments were carried out with P sufficient (P+) and total P deficient (P-) treatments using common bean SFB-1 seed cultivar. The plants were grown in MGRL media for a duration of three weeks. Various morphological traits were significantly reduced under P- stress conditions. There was a significant decrease in the levels of biochemical parameters such as free amino acids, secondary metabolites, and osmolytes (proline, total soluble sugars, and glycine betaine) under P- growth conditions. Additionally, photosynthetic pigments (such as chlorophyll a, chlorophyll b, total chlorophyll, and total carotenoids), physiological parameters (such as relative water content and stress injury), as well as both enzymatic and non-enzymatic antioxidants (including catalase, peroxidase, superoxide dismutase, total phenolics, and flavonoids), were also significantly reduced. Proteomic analysis identified two key proteins, homocysteine methyltransferase and DDB1-CUL4 associated factor 1-like protein, which are involved in various stress adaptation pathways. Furthermore, techniques such as FTIR spectroscopy, SEM, SEM-EDX, and intrinsic fluorescence spectroscopy were used to validate the metabolic changes induced by phosphorus deficiency. Phosphorus deficiency led to profound metabolic changes affecting photosynthesis, defense-related protein expression, cell wall composition, secondary metabolite production, levels of phytohormone precursors, free amino acids, antioxidant activity, and osmolyte accumulation, all of which contributed to inhibited plant growth and development.
普通菜豆（Phaseolus vulgaris）因其近乎完美的营养成分，是人类食用的最重要的豆类作物之一。然而，在所有种植系统中，作物产量总是受到磷（P）胁迫的限制。本研究旨在采用综合多组学方法，揭示普通菜豆在营养生长早期对磷胁迫的响应机制。使用普通菜豆SFB - 1种子品种进行了磷充足（P +）和总磷缺乏（P -）处理的体外实验。植株在MGRL培养基中生长三周。在P -胁迫条件下，各种形态性状显著降低。在P -生长条件下，游离氨基酸、次生代谢物和渗透调节物质（脯氨酸、总可溶性糖和甘氨酸甜菜碱）等生化参数水平显著下降。此外，光合色素（如叶绿素a、叶绿素b、总叶绿素和总类胡萝卜素）、生理参数（如相对含水量和胁迫伤害）以及酶促和非酶促抗氧化剂（包括过氧化氢酶、过氧化物酶、超氧化物歧化酶、总酚类和黄酮类）也显著降低。蛋白质组学分析鉴定出两种关键蛋白，即同型半胱氨酸甲基转移酶和类DDB1 - CUL4相关因子1蛋白，它们参与各种胁迫适应途径。此外，还使用了傅里叶变换红外光谱、扫描电子显微镜、扫描电子显微镜 - 能谱仪和内在荧光光谱等技术来验证磷缺乏引起的代谢变化。磷缺乏导致了深刻的代谢变化，影响光合作用、防御相关蛋白表达、细胞壁组成、次生代谢物产生、植物激素前体水平、游离氨基酸、抗氧化活性和渗透调节物质积累，所有这些都导致了植物生长发育受到抑制。
