与内质网相关的E2-E3泛素酶对通过调节乙烯受体的周转来调控乙烯反应。

ER-related E2-E3 ubiquitin enzyme pair regulates ethylene response by modulating the turnover of ethylene receptors.

作者信息

Zhao Qingcui, Zhou Xiaofeng, Chen Qian, Yang Ruoyun, Li Yonghong, Zhao Jiaxin, Fu Xijia, Lin Yan, Liu Yuming, Zhao Liangjun, Jiang Cai-Zhong, Gan Su-Sheng, Xie Qi, Gao Junping, Ma Nan

机构信息

Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing, 100193, China.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

出版信息

Nat Commun. 2025 Jul 1;16(1):5937. doi: 10.1038/s41467-025-61066-9.

DOI:10.1038/s41467-025-61066-9

PMID:40593775

Abstract

Gaseous phytohormone ethylene regulates various aspects of plant development. Ethylene is perceived by ER membrane-localized receptors, which are inactivated upon binding with ethylene molecules, thereby initiating ethylene signal transduction. Here, we report that a novel E3 ligase RING finger for Ethylene receptor Degradation (RED) and its E2 partner UBC32 ubiquitinate ethylene-bound receptors for degradation through an ER associated degradation (ERAD) pathway in both Rosa hybrida and Solanum lycopersicum. The depletion of RED or UBC32 leads to hypersensitivity to ethylene, which is manifested as premature leaf abscission and petal shedding in roses, as well as the dwarf plants and accelerated fruit ripening in tomatoes. Disruption of the conserved ethylene binding site of receptors prevents RED-mediated degradation of the receptors. Our study discovers an ERAD branch that facilitates the ethylene-induced degradation of receptors, and provides insights into how the plant's response to ethylene can be controlled by modulating the turnover of ethylene receptors.

摘要

气态植物激素乙烯调节植物发育的各个方面。乙烯由内质网（ER）膜定位的受体感知，这些受体在与乙烯分子结合后失活，从而启动乙烯信号转导。在此，我们报告一种新型E3连接酶——乙烯受体降解的环状结构域蛋白（RED）及其E2伴侣UBC32，它们通过内质网相关降解（ERAD）途径使结合乙烯的受体泛素化以进行降解，该过程在玫瑰和番茄中均有发生。RED或UBC32的缺失导致对乙烯超敏，在玫瑰中表现为叶片过早脱落和花瓣掉落，在番茄中则表现为植株矮小和果实成熟加速。受体保守乙烯结合位点的破坏会阻止RED介导的受体降解。我们的研究发现了一个ERAD分支，该分支促进乙烯诱导的受体降解，并为如何通过调节乙烯受体的周转来控制植物对乙烯的反应提供了见解。