Cai Mengrui, Hou Guangjian, Guo Shousen, Liu Yongxuan, Zhao Chen, Zhao Lin, Su Le, Li Kunlun, Yue Qiulin, Zhang Song
State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, P.R. China.
Jinan Hangchen Biotechnology Co., Ltd., Jinan, 250002, China.
Sci Rep. 2025 Jul 2;15(1):22534. doi: 10.1038/s41598-025-06935-5.
The seaweed Laminaria japonica possesses a wide range of nutritional and medicinal properties, such as antioxidant and hypoglycemic effects. Previous research has demonstrated that fermenting Laminaria japonica with S. cerevisiae and Lactobacillus enhances its bioactivities. In this study, fermented Laminaria japonica underwent ethanol extraction to recover bioactive compounds. The impact of fermented seaweed extracts (FSE) on renal injury in streptozotocin-induced diabetic nephropathy rats was examined. Through UHPLC-MS/MS analysis, 44 compounds were identified in the fermented seaweed extracts, with carbohydrates and organic acids being the main classes. Fermented seaweed extracts significantly reduced streptozotocin-induced hyperglycemia, improved renal function markers (BUN, SCr, and Umalb), and mitigated kidney morphological changes. Specifically, the FSE-H group exhibited a 49% reduction in fasting glucose levels compared to the model group. Additionally, fermented seaweed extracts treatment downregulated both the transcriptional and protein levels of PI3K, Akt, and mTOR, suggesting involvement of the PI3K/AKT/mTOR-regulated pathway. These findings suggest that fermented seaweed extracts have potential as a therapeutic agent or functional ingredient for the treatment of diabetic nephropathy.
海带具有广泛的营养和药用特性，如抗氧化和降血糖作用。先前的研究表明，用酿酒酵母和乳酸菌发酵海带可增强其生物活性。在本研究中，对发酵海带进行乙醇提取以回收生物活性化合物。研究了发酵海藻提取物（FSE）对链脲佐菌素诱导的糖尿病肾病大鼠肾损伤的影响。通过超高效液相色谱-串联质谱分析，在发酵海藻提取物中鉴定出44种化合物，主要类别为碳水化合物和有机酸。发酵海藻提取物显著降低了链脲佐菌素诱导的高血糖，改善了肾功能指标（血尿素氮、血清肌酐和尿微量白蛋白），并减轻了肾脏形态学变化。具体而言，与模型组相比，FSE-H组空腹血糖水平降低了49%。此外，发酵海藻提取物处理下调了PI3K、Akt和mTOR的转录和蛋白水平，表明PI3K/AKT/mTOR调节通路参与其中。这些发现表明，发酵海藻提取物有潜力作为治疗糖尿病肾病的治疗剂或功能成分。
