Wang Lilong, Wang Linlin, Yan Chunhong, Ai Chunqing, Wen Chengrong, Guo Xiaoming, Song Shuang
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Foods. 2022 Aug 8;11(15):2381. doi: 10.3390/foods11152381.
The present study aimed to clarify the potential mechanism of fucoidans found in on anti-inflammation and to further explore the relationship between their structures and anti-inflammation. Two novel fucoidans named ANP-6 and ANP-7 and found in , were separated and purified and their structures were elucidated by HPGPC, HPLC, GC-MS, FT-IR, NMR, and by the Congo red test. They both possessed a backbone constructed of →2)-α-L-Fuc4-(1→, →3)-α-L-Fuc24-(1→, →6)-β-D-Gal-(1→, and →3,6)-β-D-Gal4-(1→ with branches of →2)-α-L-Fuc4-(1→ and →3)-β-D-Gal-(1→. Moreover, ANP-6 and ANP-7 could prevent the inflammation of the LPS-stimulated macrophages by suppressing the NO production and by regulating the expressions of iNOS, COX-2, TNF-α, IL-1β, IL-6, and IL-10. Their inhibitory effects on the TLR-2 and TLR-4 levels suggest that they inhibit the inflammation process via the blocking of the TLR/NF-κB signal transduction. In addition, ANP-6, with a molecular weight (63.2 kDa), exhibited stronger anti-inflammatory capabilities than ANP-7 (124.5 kDa), thereby indicating that the molecular weight has an influence on the anti-inflammatory effects of fucoidans.
本研究旨在阐明[来源未提及]中发现的岩藻聚糖的抗炎潜在机制，并进一步探索其结构与抗炎作用之间的关系。从[来源未提及]中分离并纯化出两种名为ANP - 6和ANP - 7的新型岩藻聚糖，通过高效凝胶渗透色谱法（HPGPC）、高效液相色谱法（HPLC）、气相色谱 - 质谱联用仪（GC - MS）、傅里叶变换红外光谱仪（FT - IR）、核磁共振仪（NMR）以及刚果红试验对其结构进行了阐明。它们均具有由→2)-α-L-Fuc4-(1→、→3)-α-L-Fuc24-(1→、→6)-β-D-Gal-(1→和→3,6)-β-D-Gal4-(1→构建的主链，并带有→2)-α-L-Fuc4-(1→和→3)-β-D-Gal-(1→的分支。此外，ANP - 6和ANP - 7可通过抑制一氧化氮（NO）生成以及调节诱导型一氧化氮合酶（iNOS）、环氧化酶 - 2（COX - 2）、肿瘤坏死因子 - α（TNF - α）、白细胞介素 - 1β（IL - 1β）、白细胞介素 - 6（IL - 6）和白细胞介素 - 10（IL - 10）的表达来预防脂多糖（LPS）刺激的巨噬细胞炎症。它们对Toll样受体2（TLR - 2）和Toll样受体4（TLR - 4）水平的抑制作用表明，它们通过阻断TLR/核因子κB（NF - κB）信号转导来抑制炎症过程。此外，分子量为63.2 kDa的ANP - 6表现出比分子量为124.5 kDa的ANP - 7更强的抗炎能力，从而表明分子量对岩藻聚糖的抗炎作用有影响。
