Zhang Ruiya, Wang Lei, Zhang Rongjia, Zhang Xinyi, Huang Jianfei, Li Xuemin, Wang Bochu, Piao Shuguang, Zhou Xiaorong, Meng Run
Department of Immunology, Medical School, Nantong University, Nantong, 226001, China.
Department of Clinical Biobank & Institute of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
Sci Rep. 2025 Jul 1;15(1):21825. doi: 10.1038/s41598-025-06717-z.
The challenges associated with wound healing are multifaceted, encompassing factors such as susceptibility to infection, inadequate blood supply to injured tissues, and the retention of foreign bodies. Development of a wound repair product that can effectively overcome the aforementioned issues at a relatively low cost would better meet the needs of patients. Consequently, this research aimed to develop a low-cost hydrogel with a simple preparation process to accelerate wound healing and reduce the risk of infection. Given their abundance and low cost, ovalbumin (OVA), dendrobium polysaccharide (DOPs), and erythromycin (EM) were selected as the primary components for constructing the composite hydrogel. In vitro experiments revealed that a solution containing 0.4 g/mL OVA, 50 mg/mL DOPs, and 100 µg/mL EM could effectively form a composite hydrogel when incubated in a warm bath at 53°C for 40 min. The resulting OVA/EM/DOPs hydrogel demonstrated exceptional properties, including strong adhesion, regenerative capacity, water retention, hydrophilicity, non-hemolytic behavior, and antimicrobial activity. Cellular assays further confirmed that the OVA/EM/DOPs hydrogel exhibited low cytotoxicity, excellent biocompatibility, and the ability to enhance scratch closure in L929 cells. In vivo wound healing experiments demonstrated that the composite hydrogel significantly accelerated wound repair by upregulating the expression of CD31 and VEGF while reducing levels of IL-10 and TNF-α. Both in vitro and in vivo findings consistently supported the hydrogel's efficacy in promoting wound healing and mitigating inflammation, highlighting its considerable potential for clinical wound management. The research not only offers a promising, low-cost option for wound repair but also broadens the potential applications of DOPs. Furthermore, the successful design of this composite hydrogel provides a novel framework for developing other simple and economical hydrogel-based materials, paving the way for innovative approaches in wound care and beyond.
伤口愈合面临的挑战是多方面的，包括易受感染、受伤组织血液供应不足以及异物残留等因素。开发一种能够以相对较低成本有效克服上述问题的伤口修复产品，将更好地满足患者需求。因此，本研究旨在开发一种制备过程简单的低成本水凝胶，以加速伤口愈合并降低感染风险。鉴于卵清蛋白（OVA）、铁皮石斛多糖（DOPs）和红霉素（EM）来源丰富且成本低廉，它们被选为构建复合水凝胶的主要成分。体外实验表明，含有0.4 g/mL OVA、50 mg/mL DOPs和100 μg/mL EM的溶液在53°C温浴中孵育40分钟时可有效形成复合水凝胶。所得的OVA/EM/DOPs水凝胶具有优异的性能，包括强粘附性、再生能力、保水性、亲水性、非溶血行为和抗菌活性。细胞实验进一步证实，OVA/EM/DOPs水凝胶具有低细胞毒性、优异的生物相容性以及增强L929细胞划痕闭合的能力。体内伤口愈合实验表明，复合水凝胶通过上调CD31和VEGF的表达，同时降低IL-10和TNF-α的水平，显著加速了伤口修复。体外和体内研究结果均一致支持该水凝胶在促进伤口愈合和减轻炎症方面的功效，突出了其在临床伤口管理中的巨大潜力。该研究不仅为伤口修复提供了一种有前景的低成本选择，还拓宽了DOPs的潜在应用。此外，这种复合水凝胶的成功设计为开发其他简单且经济的水凝胶基材料提供了一个新框架，为伤口护理及其他领域的创新方法铺平了道路。
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