Zhao Yong, Gao Jie, Wang Xiaohong, Rashid Muhammad Abdul Rehman, Wu Zewen, Ma Zhiqi, Wu Hui, Xu Bingxia, Wu Zhenyuan, Gu Yunsong, Pan Yinghua, Li Danting, Wang Ruiying, Guo Zhenhua, Ma Wendong, Sun Xingming, Li Jinjie, Zhang Hongliang, Mao Tonglin, Zhang Zhanying, Li Zichao
Sanya Institute of China Agricultural University, Sanya, Hainan, China.
Hainan Seed Industry Laboratory, Sanya, Hainan, China.
Nat Commun. 2025 Jul 1;16(1):5894. doi: 10.1038/s41467-025-60604-9.
Culm diameter directly affects lodging and yield traits in cereal crops. However, the underlying molecular mechanisms of these interrelated, complex agronomic traits remain unclear. Here, we identify a quantitative trait locus for culm diameter in rice (Oryza sativa) and cloned the candidate gene, STRONG1. This gene encodes MICROTUBULE-ASSOCIATED PROTEIN 70 (MAP70), which localizes to cortical microtubules and alters the arrangement of the microtubule skeleton. Knockout or knockdown of STRONG1 enhances grain yield by synchronously improving lodging resistance, panicle architecture, and plant architecture. One single-nucleotide polymorphism, SNP - 1304 (C to A), in a MYB61-binding site within the STRONG1 promoter affects its expression, resulting in changes in cellulose content and sclerenchyma cell wall development. Rice accessions harboring the Hap-STRONG1 haplotype derived from wild rice, with reduced STRONG1 expression, show enhanced lodging resistance and yield, compared to accessions carrying Hap-STRONG1. Knockout of STRONG1 results in a 9.3-15.4% increase in yield, compared to the wild type in a field plot trial. Knockout of STRONG1 also improves panicle and plant architecture, facilitating high-density planting. This study provides a candidate gene for the development of improved rice varieties with stable, high yields.
茎粗直接影响谷类作物的倒伏和产量性状。然而，这些相互关联的复杂农艺性状的潜在分子机制仍不清楚。在这里，我们鉴定了水稻（Oryza sativa）茎粗的一个数量性状位点，并克隆了候选基因STRONG1。该基因编码微管相关蛋白70（MAP70），其定位于皮层微管并改变微管骨架的排列。敲除或敲低STRONG1通过同步提高抗倒伏性、穗部结构和植株结构来提高谷物产量。STRONG1启动子内一个MYB61结合位点的单核苷酸多态性SNP - 1304（C到A）影响其表达，导致纤维素含量和厚壁组织细胞壁发育发生变化。与携带Hap-STRONG1单倍型的材料相比，含有源自野生稻的Hap-STRONG1单倍型且STRONG1表达降低的水稻材料表现出更强的抗倒伏性和产量。在田间小区试验中，与野生型相比，敲除STRONG1导致产量提高9.3 - 15.4%。敲除STRONG1还改善了穗部和植株结构，有利于高密度种植。本研究为培育具有稳定高产的改良水稻品种提供了一个候选基因。
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