Zhou Guanghong, Tao Min, Zhu Jie, Li Shengbing, Zhang Lili
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China.
The Second Affiliated Hospital, Kunming Medical University, Kunming, 650021, China.
Sci Rep. 2025 Jul 2;15(1):23154. doi: 10.1038/s41598-025-04665-2.
Hypoxia preconditioned plasma (HPP) is generated by cultivating blood under hypoxic conditions, allowing blood cells to naturally regulate the production of growth factors in response to hypoxia, which seems ideal for diabetic wound treatment. This study aims to verify the efficacy and underlying mechanism of HPP on diabetic wound healing. The concentrations of angiogenesis-related factors (VEGF, PDGF, EGF, IGF, TSP1, PF4) measured by ELISA were significantly increased in HPP. In vitro, HPP promoted the proliferation and migration of HUVECs and HSF, and enhanced the tube formation of HUVECs. In vivo, HPP promotes wound angiogenesis and collagen deposition, significantly accelerating diabetic wound healing (84 ± 10% vs. 65 ± 20%, p < 0.01). Furthermore, western blot and qPCR results showed that both in cells and wounds, the expression of pro-angiogenic factors, as well as p-AKT, was increased in HPP. These findings suggest that HPP benefits diabetic wound healing and may represent a promising therapeutic approach for diabetic wound healing.
缺氧预处理血浆（HPP）是通过在缺氧条件下培养血液产生的，使血细胞能够自然调节对缺氧作出反应的生长因子的产生，这似乎是糖尿病伤口治疗的理想选择。本研究旨在验证HPP对糖尿病伤口愈合的疗效及潜在机制。通过酶联免疫吸附测定法（ELISA）检测发现，HPP中血管生成相关因子（VEGF、PDGF、EGF、IGF、TSP1、PF4）的浓度显著增加。在体外，HPP促进人脐静脉内皮细胞（HUVECs）和人皮肤成纤维细胞（HSF）的增殖和迁移，并增强HUVECs的管腔形成。在体内，HPP促进伤口血管生成和胶原蛋白沉积，显著加速糖尿病伤口愈合（84±10%对65±20%，p<0.01）。此外，蛋白质印迹法和定量聚合酶链反应（qPCR）结果表明，在细胞和伤口中，HPP中促血管生成因子以及磷酸化蛋白激酶B（p-AKT）的表达均增加。这些发现表明，HPP有益于糖尿病伤口愈合，可能是一种有前景的糖尿病伤口愈合治疗方法。
