Li Xiaodan, Jia Fang, Bai Huiwen, Chen Xuejing, Zhang Yue, Zhang Ningfang, Yang Hongxin
College of Basic Medicine, Inner Mongolia Medical University, Hohhot, China.
First Clinical Medical College, Inner Mongolia Medical University, Hohhot, China.
Sci Rep. 2025 Jul 1;15(1):21506. doi: 10.1038/s41598-025-08530-0.
Estrogen is used for endometrial repair; however, it has limited effectiveness. Cytokine S100A8 expression is affected by estrogen and is involved in regulating the damage repair. We aimed to confirm the effects of S100A8 and explore its mechanisms during the reconstruction of the injured endometrium. We investigated the effects of estrogen on S100A8 expression in healthy endometrium. A rat model of endometrial injury and cultured primary endometrial cells were used to determine the pleiotropic effects of S100A8 on endometrial epithelial repair. Estrogen regulated the recruitment of S100A8-positive immune cells (S100A8-PICs) and the release of S100A8 in the healthy endometrium, but estrogen plays a limited role in regulating seriously injured endometrium via self-S100A8. S100A8 administration to the uterine cavity significantly improved the morphology of injured epithelium; influenced the reverse migration of S100A8-PICs; and promoted epithelial localization of proliferating cells, cell junction formation, and transformation of stromal cells to epithelial cells. S100A8 in the uterine cavity has pleiotropic effects that improve endometrial epithelial reconstruction and can compensate for the deficiency in estrogen repair regulation.
雌激素用于子宫内膜修复；然而，其效果有限。细胞因子S100A8的表达受雌激素影响，并参与调节损伤修复。我们旨在确认S100A8的作用，并探索其在受损子宫内膜重建过程中的机制。我们研究了雌激素对健康子宫内膜中S100A8表达的影响。利用大鼠子宫内膜损伤模型和原代培养的子宫内膜细胞，确定S100A8对子宫内膜上皮修复的多效性作用。雌激素调节健康子宫内膜中S100A8阳性免疫细胞（S100A8-PICs）的募集和S100A8的释放，但雌激素通过自身S100A8对严重受损子宫内膜的调节作用有限。向子宫腔内施用S100A8可显著改善受损上皮的形态；影响S100A8-PICs的逆向迁移；并促进增殖细胞的上皮定位、细胞连接形成以及基质细胞向上皮细胞的转化。子宫腔内的S100A8具有多效性作用，可改善子宫内膜上皮重建，并可弥补雌激素修复调节的不足。
