Bianchi P, Jacques C, Theunis J, Jamin E L, Orlandi C, Cauhape L, Alvarez-Georges S, Alves A, Simcic-Mori A, Lauze C, Gravier E, Carballido F, Ribet V, Bessou-Touya S, Duplan H
Pierre Fabre Dermo-Cosmetics & Personal Care, Centre R&D Pierre Fabre, Toulouse, 31025, France.
UMR1331 Toxalim (Research Centre in Food Toxicology), Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.
Sci Rep. 2025 Jul 1;15(1):22282. doi: 10.1038/s41598-025-07547-9.
We investigated changes in skin microbiome and metabolome linked to wound healing and how these are affected by a formula known to improve re-epithelialization. In a clinical study with 21 subjects, forearm lesions were induced by epidermal laser ablation. The areas were left untreated or treated with the formula. Re-epithelialization was monitored for 18 days. Skin swabs were analyzed for microbiome diversity using 16 S rRNA gene sequence analysis. Selected species analyzed using digital droplet polymerase chain reaction. Metabolomic profiles were analyzed by ultra-high performance liquid chromatography-high-resolution mass spectrometry. Microbiota alpha-diversity (richness and evenness) was markedly reduced by laser ablation and returned to pre-ablation levels on Day 5. Formula application accelerated the re-epithelialization time (RT), which was more efficient for slow healing (RTs of 15-19 days) than quick healing (10-12 day RTs) subjects. The repairing effect was associated with greater microbiota diversity and species-specific growth of commensal bacteria. Levels of several metabolites on untreated skin at the RT and the extent of the impact of the formula were different in slow and quick healers. The formula significantly modified the skin metabolome, whereby metabolites involved in promoting wound healing were increased and metabolites consumed by the commensal bacteria were decreased.
我们研究了与伤口愈合相关的皮肤微生物组和代谢组的变化，以及一种已知可促进再上皮化的配方对这些变化的影响。在一项针对21名受试者的临床研究中，通过表皮激光消融诱导前臂损伤。这些区域不进行治疗或用该配方进行治疗。监测再上皮化过程18天。使用16S rRNA基因序列分析对皮肤拭子进行微生物组多样性分析。使用数字液滴聚合酶链反应分析选定的物种。通过超高效液相色谱-高分辨率质谱分析代谢组学图谱。激光消融显著降低了微生物群的α多样性（丰富度和均匀度），并在第5天恢复到消融前水平。应用配方加速了再上皮化时间（RT），对于愈合缓慢（RT为15 - 19天）的受试者比愈合迅速（RT为10 - 12天）的受试者更有效。修复效果与更大的微生物群多样性和共生细菌的物种特异性生长有关。在RT时，未治疗皮肤上几种代谢物的水平以及配方的影响程度在愈合缓慢和迅速的受试者中有所不同。该配方显著改变了皮肤代谢组，从而增加了参与促进伤口愈合的代谢物，并减少了共生细菌消耗的代谢物。
