Granghelli Carlos Alexandre, Lopes Mário Henrique Scapin, Rangel Luís, Campbell Joy, Polo Javier, Crenshaw Joe, Celeghini Eneiva Carla Carvalho, Jorge-Neto Pedro Nacib, Ferreira Helena Lage, Moura Vinicius Santos, Motta Jennifer Soutter, Araujo Cristiane Soares da Silva, Araujo Lucio Francelino
Departamento de Zootecnia, Faculdade de Zootecnia e Engenharia de Alimentos - FZEA, Universidade de São Paulo - USP, Pirassununga, SP, Brasil.
APC, LLC, Ankeny, IA, USA.
Anim Reprod. 2025 Jun 30;22(2):e20240111. doi: 10.1590/1984-3143-AR2024-0111. eCollection 2025.
A total of 216 Cobb 500 broiler breeder hens were randomly distributed across two treatments consisting of diets supplemented with 0 or 1% spray-dried plasma (SDP), resulting in 27 replications per treatment and four birds per pen. In addition, 36 roosters, housed in individual pens, were allocated to the same treatments, with each bird considered a replicate, in order to assess the influence of SDP on semen and hatching characteristics. The experimental diets were fed from 26 weeks until the conclusion of the study, at 65 weeks of age. Semen quality, embryonic mortality and quality of newly hatched chicks were evaluated at 29, 45, and 63 weeks. Hens were inseminated with fresh semen, and eggs were incubated following standard procedures. Semen from 63-week-old roosters had higher sperm concentration than other age groups, while 29-week-old rooster semen exhibited higher progressive motility than semen from older roosters ( < 0.001). The 45-week-old roosters had the lowest values for the analyzed semen quality parameters (average path velocity, straight-line velocity and curvilinear velocity). Additionally, sperm from 45-week-old roosters, regardless of SDP supplementation, had fewer total morphological defects than 63-week-old roosters. However, 1% SDP in the diet reduced total sperm cell defects at 63 weeks of age, as well as total sperm head and tail defects ( < 0.05) compared to unsupplemented birds. Dietary SDP reduced late embryonic death in 63-week-old breeders ( < 0.05). The results demonstrate that 1% SDP in breeder diets reduced late embryonic mortality and sperm cell defects, mainly in aged birds, enhancing the reproductive performance of broiler breeders.
总共216只科布500肉种母鸡被随机分配到两种处理组，分别饲喂添加0%或1%喷雾干燥血浆（SDP）的日粮，每个处理组有27个重复，每栏4只鸡。此外，36只公鸡单独饲养在单栏中，分配到相同的处理组，每只鸡作为一个重复，以评估SDP对精液和孵化特性的影响。实验日粮从26周龄开始饲喂，直至65周龄实验结束。在29、45和63周龄时评估精液质量、胚胎死亡率和新孵出雏鸡的质量。用新鲜精液对母鸡进行人工授精，并按照标准程序孵化鸡蛋。63周龄公鸡的精液精子浓度高于其他年龄组，而29周龄公鸡精液的前向运动性高于年龄较大公鸡的精液（P<0.001）。45周龄公鸡的精液质量参数（平均路径速度、直线速度和曲线速度）值最低。此外，无论是否添加SDP，45周龄公鸡的精子总形态缺陷都比63周龄公鸡少。然而，与未添加SDP的鸡相比，日粮中添加1%SDP可降低63周龄时精子细胞的总缺陷以及精子头部和尾部的总缺陷（P<0.05）。日粮中添加SDP可降低63周龄种鸡的晚期胚胎死亡率（P<0.05）。结果表明，种鸡日粮中添加1%SDP可降低晚期胚胎死亡率和精子细胞缺陷，主要是在老龄鸡中，提高了肉种鸡的繁殖性能。
