Mohammadzadeh Roghayeh, Habibi Mehri, Asadi Karam Mohammad Reza
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
Future Microbiol. 2025 Aug;20(11):723-733. doi: 10.1080/17460913.2025.2525711. Epub 2025 Jul 2.
Urinary tract infections (UTIs) caused by are common infectious diseases. Currently, no vaccine is available against UTIs, making the development of an effective vaccine a critical goal to reduce the prevalence and antibiotic resistance of UTIs. Hence, we have investigated the immunogenicity and protective potential of a bivalent vaccine candidate comprising the iron scavenger receptors PMI1426 and PMI1945 from in mice.
In this study, vaccine candidates composed of PMI1426 and PMI1945 from were developed, and their immunogenicity, efficacy, and synergistic effects were evaluated in mice.
Serum levels of IgG, IgG isotypes, IgA, and urinary IgA, as well as cytokine levels including IFN-γ, IL-4, and IL-17 were significantly elevated following vaccination with PMI1426 and PMI1945 as compared to the controls. The addition of alum enhanced the immune responses in both single and combined vaccine formulations. Furthermore, bacterial colonization in the bladder of mice challenged with was significantly reduced in mice received the proteins admixed with alum compared to control groups.
Our results support the development of a promising vaccine candidate against UTIs. Future human clinical trials are needed to validate the efficacy and safety of the vaccine candidates.
由[具体病原体未提及]引起的尿路感染（UTIs）是常见的传染病。目前，尚无针对UTIs的疫苗，因此开发一种有效的疫苗成为降低UTIs患病率和抗生素耐药性的关键目标。因此，我们研究了一种包含来自[具体来源未提及]的铁清除受体PMI1426和PMI1945的二价候选疫苗在小鼠中的免疫原性和保护潜力。
在本研究中，开发了由来自[具体来源未提及]的PMI1426和PMI1945组成的候选疫苗，并在小鼠中评估了它们的免疫原性、功效和协同作用。
与对照组相比，用PMI1426和PMI1945接种疫苗后，血清IgG、IgG亚型、IgA和尿IgA水平以及包括IFN-γ、IL-4和IL-17在内的细胞因子水平显著升高。添加明矾增强了单一和联合疫苗制剂中的免疫反应。此外，与对照组相比，在接受与明矾混合的蛋白质的小鼠中，用[具体病原体未提及]攻击的小鼠膀胱中的细菌定植显著减少。
我们的结果支持开发一种有前景的针对[具体病原体未提及]UTIs的候选疫苗。未来需要进行人体临床试验以验证候选疫苗的疗效和安全性。
