Chen Wei, Zhang Ziqi, Bai Rujin, Li Siting, Chen Zhou, Ma Aijin, Jia Yingmin
School of Food and Health, Beijing Technology and Business University (BTBU), Beijing 100048, PR China.
J Agric Food Chem. 2025 Jul 16;73(28):17776-17787. doi: 10.1021/acs.jafc.5c03843. Epub 2025 Jul 2.
α-L-Arabinofuranosidases (ABFs) are essential accessory enzymes that remove arabinose side chains from arabinoxylans and work synergistically with xylanases to deconstruct complex hemicelluloses. Two GH62 ABFs, TsAbf62A and TsAbf62B, from were cloned and expressed in . The recombinant enzymes, rTsAbf62A and rTsAbf62B, exhibited strong acidophilic properties, with optimal activity at pH 3.0, retaining over 50% activity across pH 2.0-8.0 and 2.0-7.0, respectively. Both ABFs were cold-adapted, with temperature optima of 40 and 45 °C, maintaining 35.1 and 28.9% activity at 10 °C. They displayed high specificity toward wheat arabinoxylan, with specific activities of 78.99 and 11.52 U/mg, respectively. rTsAbf62A showed excellent product tolerance, retaining 85.5 and 51.9% activity in 1 M D-xylose and l-arabinose. Ca significantly enhanced thermostability, while Mn improved catalytic efficiency by modulating kinetic parameters. Circular dichroism analysis indicated both ions stabilized β-sheet structures under thermal stress. Co-incubation with a GH11 xylanase from synergistically enhanced bran hydrolysis, increasing the release of reducing sugars and phenolic compounds. These properties suggest that rTsAbf62A and rTsAbf62B are promising biocatalysts for applications in the feed and food industries.
α-L-阿拉伯呋喃糖苷酶（ABFs）是重要的辅助酶，可从阿拉伯木聚糖中去除阿拉伯糖侧链，并与木聚糖酶协同作用以解构复杂的半纤维素。从[具体来源未给出]克隆了两种GH62 ABFs，即TsAbf62A和TsAbf62B，并在[具体表达宿主未给出]中表达。重组酶rTsAbf62A和rTsAbf62B表现出很强的嗜酸特性，在pH 3.0时具有最佳活性，在pH 2.0 - 8.0和2.0 - 7.0范围内分别保留超过50%的活性。两种ABFs均适应低温，最适温度分别为40和45°C，在10°C时分别保持35.1%和28.9%的活性。它们对小麦阿拉伯木聚糖具有高度特异性，比活性分别为78.99和11.52 U/mg。rTsAbf62A表现出优异的产物耐受性，在1 M D-木糖和L-阿拉伯糖中分别保留85.5%和51.9%的活性。Ca显著提高了热稳定性，而Mn通过调节动力学参数提高了催化效率。圆二色性分析表明，在热应激下，这两种离子均稳定了β-折叠结构。与来自[具体来源未给出]的GH11木聚糖酶共同孵育可协同增强麸皮水解，增加还原糖和酚类化合物的释放。这些特性表明，rTsAbf62A和rTsAbf62B是在饲料和食品工业应用中很有前景的生物催化剂。
