Rodriguez Alexis R, Guimarães João Pedro Tôrres, Chakroborty Anand, Babcock Rachel L, Miranda Jonathan M, Kaur Gurvinder, Dufour Jannette M
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
Department of Medical Education, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
Methods Mol Biol. 2025;2954:163-182. doi: 10.1007/978-1-0716-4698-4_8.
Sertoli cells (SCs), somatic cells critical for spermatogenesis, maintain an immunoregulatory microenvironment that promotes germ cell development and migration through the blood-testis barrier and tolerance to immunogenic germ cells. Due to this unique phenotype, SCs have been studied for their role in sperm development, male fertility, and male contraception, as well as their possible use as an alternative and novel approach for use in transplantation, gene therapy, and treatment of immune-related diseases. Hence, we provide a detailed methods section, including a step-by-step protocol showing how to isolate SCs from the testis of different species, such as mice, rats, and pigs, and subsequent culturing and preparation of the isolated SCs for transplantation.
支持细胞（SCs）是精子发生所必需的体细胞，它维持着一种免疫调节微环境，这种微环境通过血睾屏障促进生殖细胞的发育和迁移，并使机体对具有免疫原性的生殖细胞产生耐受。由于这种独特的表型，支持细胞在精子发育、男性生育能力和男性避孕方面的作用以及它们作为移植、基因治疗和免疫相关疾病治疗的替代和新方法的潜在用途一直是研究的热点。因此，我们提供了一个详细的方法部分，包括一个逐步方案，展示如何从小鼠、大鼠和猪等不同物种的睾丸中分离支持细胞，以及随后对分离出的支持细胞进行培养和制备以用于移植。
