Sorensen E M, Acosta D
Toxicol Lett. 1985 Sep;27(1-3):73-82. doi: 10.1016/0378-4274(85)90122-5.
Primary cultures of rat hepatocytes were exposed to several concentrations of erythromycin estolate (EE). Hepatotoxicity was evaluated using lactate dehydrogenase (LDH) leakage and morphometric analysis of representative populations of cells examined optically. Results of the two techniques provided parallel information: cells exposed to the higher concentrations of EE had significantly greater LDH release and higher percentages of morphologically damaged cells. Planimetric analysis of a second set of hepatocytes showed increasing swelling of cells with increasing concentration of EE. Severe cellular swelling preceded disintegration, as hepatocytes became progressively more damaged by EE.
将大鼠肝细胞的原代培养物暴露于几种浓度的依托红霉素（EE）中。使用乳酸脱氢酶（LDH）泄漏和光学检查代表性细胞群体的形态计量分析来评估肝毒性。这两种技术的结果提供了平行信息：暴露于较高浓度EE的细胞具有明显更高的LDH释放和更高百分比的形态受损细胞。对另一组肝细胞的平面测量分析表明，随着EE浓度的增加，细胞肿胀加剧。随着肝细胞受到EE的损伤逐渐加重，严重的细胞肿胀先于细胞解体出现。
