Prado Andressa, Veiga Flávia Franco, de Oliveira Brito Rubens, da Cruz Alves Pereira Elton, Negri Melyssa, Svidzinski Terezinha Inez Estivalet
Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringá, Avenida Colombo, 5790- Bloco T20, Sala 203, Maringá, Paraná, 87020-900, Brazil.
Department of Diagnostic and Therapeutic Endoscopy, Mgastro Digestive Tract Medical Center, Maringa, Brazil.
Folia Microbiol (Praha). 2025 Jul 2. doi: 10.1007/s12223-025-01286-0.
This study characterized biofilms formed by Candida albicans and C. tropicalis isolated from the gastric mucosa biopsies of an intragastric balloon (IGB) patient user. Both were cultivated to form single and mixed artificial biofilms at pH 2.5 and 5.5 for 24 and 48 h. The biofilms were assessed for biochemical, metabolic, and ultrastructural properties. Yeast counts in monospecies C. albicans and C. tropicalis biofilms were higher than their respective levels in mixed biofilms across both pH values. Single C. tropicalis biofilms exhibited greater metabolic activity at both time points than C. albicans and mixed biofilms. While there were no notable pH or time-dependent differences in C. albicans and C. tropicalis monospecies biofilm formation, mixed biofilms displayed significantly higher biomass at pH 2.5. Ct also demonstrated pronounced filamentation within 24 h at pH 5.5. Scanning electron microscopy revealed cellular damage in mixed biofilms at pH 2.5; although, the biofilm structure was well developed within 24-48 h. Our findings indicate that yeasts isolated from IGB patients can form mono and polymicrobial biofilms under harsh conditions, with both species demonstrating biofilm viability at pH 2.5. Notably, C. tropicalis exhibited increased competitiveness in mixed biofilms under these conditions.
本研究对从胃内气球（IGB）使用者的胃黏膜活检样本中分离出的白色念珠菌和热带念珠菌形成的生物膜进行了表征。将这两种念珠菌分别培养，在pH 2.5和5.5条件下形成单一生物膜和混合生物膜，培养时间分别为24小时和48小时。对生物膜的生化、代谢和超微结构特性进行了评估。在两个pH值条件下，白色念珠菌和热带念珠菌单一生物膜中的酵母计数均高于其在混合生物膜中的相应水平。在两个时间点，单一热带念珠菌生物膜均比白色念珠菌和混合生物膜表现出更高的代谢活性。虽然白色念珠菌和热带念珠菌单一生物膜形成过程中不存在显著的pH或时间依赖性差异，但混合生物膜在pH 2.5时显示出显著更高的生物量。热带念珠菌在pH 5.5时24小时内也表现出明显的丝状化。扫描电子显微镜显示，pH 2.5时混合生物膜存在细胞损伤；不过，生物膜结构在24 - 48小时内发育良好。我们的研究结果表明，从IGB患者分离出的酵母能够在恶劣条件下形成单一和多微生物生物膜，两种念珠菌在pH 2.5时均表现出生物膜活力。值得注意的是，在这些条件下，热带念珠菌在混合生物膜中表现出更强的竞争力。
