噬菌体phi 6分段双链RNA基因组及其信使RNA的末端序列。

Terminal sequences of the bacteriophage phi 6 segmented dsRNA genome and its messenger RNAs.

作者信息

Szekeres M, Brownstein B H, Revel H R, Haselkorn R

出版信息

Virology. 1985 Apr 15;142(1):1-11. doi: 10.1016/0042-6822(85)90417-9.

Abstract

The ends of the three dsRNA genome segments (L, M, and S) of bacteriophage phi 6 (strand separated and/or intact) and the 5' ends of the middle and small single-strand messenger RNAs have been sequenced by base-specific partial enzymatic digestion. Terminal sequences for the large and middle dsRNA strands extend about 60 bases. The three dsRNA segments have 18 homologous bases at the left end except for position 2, which differs in the L segment. A 17-base homology defines the right ends of L and M dsRNAs and probably S dsRNA as well. The 5' ends of middle and small messenger RNAs are identical to the corresponding viral (+) strands.

摘要

通过碱基特异性部分酶切法对噬菌体φ6的三个双链RNA基因组片段（L、M和S，单链分离和/或完整）的末端以及中、小单链信使RNA的5'末端进行了测序。大双链RNA链和中双链RNA链的末端序列延伸约60个碱基。除了第2位（L片段中不同）外，三个双链RNA片段在左端有18个同源碱基。一个17碱基的同源性定义了L和M双链RNA以及可能还有S双链RNA的右端。中、小信使RNA的5'末端与相应的病毒（+）链相同。