Zhan Jiaguo, Cheng Jiale, Yang Yanan, Xu Xiaofan, Lu Zhiyi, Li Leilei, Li Haoyu, Yang Qiannan, Hu Yanran, Song Yuxin, Fan Qingmiao, Yang Enwei, Liang Qianyue, Sun Shuangyong, Qiu Feng, Cao Ying, Wu Chongming
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Jinyao Darentang Modern Chinese Medicine Industrial Park Traditional Chinese Medicine Research Institute, Tianjin, China.
Front Pharmacol. 2025 Jun 18;16:1587119. doi: 10.3389/fphar.2025.1587119. eCollection 2025.
Sanhuang refers to the three cold-natured and bitter-flavored traditional Chinese medicines, namely, Georgi (HuangQin, HQ), Franch. (HuangLian, HL), and Cortex (HuangBo, HB). Although similar in drug properties, they are traditionally used to treat different dampness-heat syndromes belonging to the Upper Jiao (lung and heart diseases), the Middle Jiao (stomach and intestine diseases), and the Lower Jiao (intestine, kidney, and bladder diseases). The mechanisms behind their differential effects remain unexplored.
A model of large intestine dampness-heat syndrome colitis was established through the administration of exogenous hygrothermal conditions combined with lipopolysaccharide (LPS) and . This model was employed to evaluate the efficacy of Cortex, Franch., and Georgi. Full-length 16S rRNA amplicon sequencing was utilized to assess changes in gut microbiota following drug interventions. Ultimately, the therapeutic effects of key microbial strains on ulcerative colitis were confirmed using a dextran sulfate sodium (DSS)-induced colitis model.
The results showed that HL and HB exhibited significant remedial effects on large intestine dampness-heat syndrome (LIDHS) colitis, but HQ did not. Gut microbial analysis revealed that HL and HB markedly shifted the overall structure of gut microbiota, while HQ showed little impact. The increase of sp. was a common sign in both HL- and HB-treated animals, but it was not observed in the HQ group. On the contrary, the abundance of a -dominant co-abundance gene group (CAG) significantly declined in the HL and HB groups but was similar to the negative control in the HQ group. Additionally, our observations indicate that the enrichment of is consistent with the difference in drug efficacy. experiments also demonstrated the anti-colitis efficacy of .
This study identifies as the key bacterium against ulcerative colitis through the establishment of a novel model and a drug comparison.
三黄指三种性寒味苦的传统中药，即黄芩（HuangQin，HQ）、黄连（HuangLian，HL）和黄柏（HuangBo，HB）。尽管它们在药性上相似，但传统上用于治疗属于上焦（肺和心脏疾病）、中焦（胃和肠道疾病）和下焦（肠道、肾脏和膀胱疾病）的不同湿热证。它们产生不同作用的机制仍未得到探索。
通过给予外源性湿热条件结合脂多糖（LPS）建立大肠湿热证结肠炎模型。该模型用于评估黄柏、黄连和黄芩的疗效。利用全长16S rRNA扩增子测序评估药物干预后肠道微生物群的变化。最终，使用葡聚糖硫酸钠（DSS）诱导的结肠炎模型证实关键微生物菌株对溃疡性结肠炎的治疗作用。
结果表明，HL和HB对大肠湿热证（LIDHS）结肠炎具有显著的治疗作用，但HQ没有。肠道微生物分析表明，HL和HB显著改变了肠道微生物群的整体结构，而HQ的影响很小。某菌属的增加是HL和HB治疗组动物的共同特征，但在HQ组未观察到。相反，HL和HB组中一个优势共丰度基因组（CAG）的丰度显著下降，但在HQ组中与阴性对照组相似。此外，我们的观察表明，某菌属的富集与药物疗效的差异一致。实验还证明了某菌的抗结肠炎疗效。
本研究通过建立新模型和药物比较，确定某菌为抗溃疡性结肠炎的关键细菌。
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