兔源不同免疫球蛋白G制剂对肠杆菌科细菌的体外和体内活性

In vitro and in vivo activities of different immunoglobulin G preparations from the rabbit against Enterobacteriaceae.

作者信息

Trautmann M, Müller-Leutloff Y, Hofstaetter T, Seiler F R, Hahn H

出版信息

Vox Sang. 1985;49(4):267-76. doi: 10.1111/j.1423-0410.1985.tb01121.x.

DOI:10.1111/j.1423-0410.1985.tb01121.x

Abstract

Specific rabbit IgG, prepared against three enterobacterial strains, was modified according to procedures used for the production of human IgG preparations suitable for intravenous infusion. The resulting products were examined for their in vitro opsonic and in vivo protective activity against the respective bacterial strains. A sulfonated IgG preparation (S-sIgG) and the enzymatically derived fragments F(ab')2 and Fab/Fc were opsonic in vitro and provided in vivo protection against lethal enterobacterial infection in mice. Protection by Fab fragments varied in the different in vivo models.

摘要

针对三种肠道细菌菌株制备的特异性兔免疫球蛋白G（IgG），按照用于生产适合静脉输注的人IgG制剂的程序进行了修饰。对所得产物进行了体外调理活性和针对相应细菌菌株的体内保护活性检测。一种磺化IgG制剂（S-sIgG）以及酶促衍生片段F(ab')2和Fab/Fc在体外具有调理作用，并在体内为小鼠提供了针对致死性肠道细菌感染的保护。Fab片段的保护作用在不同的体内模型中有所不同。