由沙丁鱼蛋白/魔芋葡甘聚糖复合物稳定的皮克林乳液凝胶递送虾青素以缓解炎症性肠病。
Pickering emulsion gels stabilized by sardine protein/konjac glucomannan complexes delivering astaxanthin for alleviating inflammatory bowel disease.
作者信息
Liang Jiayue, Ma Jiale, Sun Yu, Su Wentao, Cheng Shasha
机构信息
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Dalian Key Laboratory for Precision Nutrition, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Dalian Key Laboratory for Precision Nutrition, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
出版信息
Int J Biol Macromol. 2025 Aug;320(Pt 1):145690. doi: 10.1016/j.ijbiomac.2025.145690. Epub 2025 Jul 1.
Astaxanthin (AXT) alleviates inflammatory bowel disease (IBD) by reducing inflammation, blocking NF-κB pathway, and modulating gut microbiota. However, the poor solubility and low bioavailability of AXT limit its applications in food. In this study, sardine protein (SDP)/konjac glucomannan (KGM)-stabilized Pickering emulsion gels (SKEGs) were developed for AXT delivery for IBD intervention. Initially, the primary interactions between SDP and KGM were identified as hydrogen bonding and hydrophobic interactions. The addition of KGM effectively enhanced the interfacial adsorption capacity and wettability of SDP. Notably, at a KGM concentration of 1.2 %, the formed Pickering emulsion exhibited a more uniform and denser microstructure, with an oil droplet size of 7.12 ± 0.14 μm. Furthermore, the incorporation of konjac glucomannan (KGM) significantly improved the emulsion stability under the conditions of high temperature, high ionic strength and long-term storage. After 14 h of ultraviolet irradiation and heating, the retention rate of AXT in SKEGs was markedly higher than that of free AXT. Simulated digestion experiments revealed that SKEGs improved the bioavailability of AXT by reducing the release of AXT in stomach. In vivo mouse experiments demonstrated that the AXT-loaded SKEGs effectively alleviated weight loss and colon shortening induced by dextran sodium sulfate, and protected the integrity of colon tissue by regulating inflammatory factor levels s and restoring the expression of tight junction proteins including ZO-1 and occludin. Additionally, the AXT-loaded SKEGs restored the gut microbiota composition in IBD mice by reducing harmful bacteria and enriching beneficial species. This study presented a novel strategy for improving the biological accessibility of AXT in functional food formulations, simultaneously facilitating the efficient utilization of ocean resources.
虾青素（AXT）通过减轻炎症、阻断核因子-κB（NF-κB）信号通路和调节肠道微生物群来缓解炎症性肠病（IBD）。然而，AXT的低溶解度和低生物利用度限制了其在食品中的应用。在本研究中，开发了沙丁鱼蛋白（SDP）/魔芋葡甘聚糖（KGM）稳定的Pickering乳液凝胶（SKEGs）用于AXT递送以干预IBD。首先，确定SDP和KGM之间的主要相互作用为氢键和疏水相互作用。KGM的添加有效提高了SDP的界面吸附能力和润湿性。值得注意的是，在KGM浓度为1.2%时，形成的Pickering乳液呈现出更均匀、更致密的微观结构，油滴尺寸为7.12±0.14μm。此外，魔芋葡甘聚糖（KGM）的加入显著提高了乳液在高温、高离子强度和长期储存条件下的稳定性。经过14小时的紫外线照射和加热后，AXT在SKEGs中的保留率明显高于游离AXT。模拟消化实验表明，SKEGs通过减少AXT在胃中的释放提高了AXT的生物利用度。体内小鼠实验表明，负载AXT的SKEGs有效减轻了葡聚糖硫酸钠诱导的体重减轻和结肠缩短，并通过调节炎症因子水平和恢复包括闭锁小带蛋白1（ZO-1）和闭合蛋白在内的紧密连接蛋白的表达来保护结肠组织的完整性。此外，负载AXT的SKEGs通过减少有害细菌并富集有益菌种恢复了IBD小鼠的肠道微生物群组成。本研究提出了一种新的策略，可提高AXT在功能性食品配方中的生物可及性，同时促进海洋资源的高效利用。
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