Naylor J W, Miller W G, Stokes G N, Stott G G
Am J Phys Anthropol. 1985 Oct;68(2):197-200. doi: 10.1002/ajpa.1330680206.
Cemental annulations are easily countable in teeth from animals that have an exaggerated regular change of food intake from season to season. Although present in human teeth, cemental annulations are not always easy to count. A method for preparing human teeth for evaluation involving collection, identification, measuring, sectioning, cleaning, acid etching, staining, and mounting is reported. Sections 100-microns thick were stained with cresyl fast violet as a stain of choice and were photographed using standard light microscopic techniques as well as Nomarsky interference microscopy. Countability of annulations from photographic enlargements was evaluated.
在食物摄入量随季节有明显规律变化的动物牙齿中，牙骨质环纹很容易计数。虽然人类牙齿中也存在牙骨质环纹，但并不总是易于计数。本文报道了一种用于评估人类牙齿的制备方法，该方法包括收集、识别、测量、切片、清洁、酸蚀、染色和装片。将100微米厚的切片用甲酚紫作为首选染色剂进行染色，并使用标准光学显微镜技术以及诺马斯基干涉显微镜进行拍照。评估了从放大照片上计数环纹的可行性。
