Seresht Parvin Mirzaei, Amini Abdollah, Pourmasoumi Parvin, Hajihosseintehrani Masoumeh, Ahmadi Houssein, Fallah Bahareh, Zare Fatemeh, Bayat Soroush, Chien Sufan, Albright Richard, Bayat Mohammad
Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Islamic Republic of Iran.
Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, 19395-1495, Tehran, Islamic Republic of Iran.
Lasers Med Sci. 2025 Jul 5;40(1):308. doi: 10.1007/s10103-025-04559-9.
Photobiomodulation (PBM), which stimulates cellular functions through light emission, has recently attracted significant attention in regenerative medicine. This study explores the effects of PBM with two wavelengths on human adipose-derived stem cells (ADSCs) and the characteristics of their secreted exosomes in vitro. ADSCs were isolated from human adipose tissue and treated with PBM with 650 nm and 810 nm wavelengths. The therapeutic effects, including cell viability, proliferation, and exosome characteristics, were evaluated using MTT assays, scanning electron microscopy (SEM), BCA assays to determine exosomal protein concentration, and Dynamic Light Scattering (DLS) to assess exosome size distribution. The results indicated that PBM at both wavelengths significantly enhanced ADSCs viability and proliferation, as confirmed by the MTT assay (p = 0.008 for 650 nm laser and p = 0.007 for 810 nm laser). Additionally, exosome analysis revealed that exosomes from PBM-treated groups exhibited a higher protein concentration and an average size of around 86 nm, as measured by BCA and DLS. We concluded that PBM with 650 nm and 810 nm wavelengths individually significantly enhances the proliferation and survival of ADSCs and improves exosome quality in vitro. The treatment increased exosomal protein concentration and improved size distribution, reflecting enhanced cell function. These findings suggest that PBM could be a promising stimulatory approach for improving ADSC-based therapies in regenerative medicine, particularly for tissue repair and chronic wound healing. Further research is needed to explore the molecular mechanisms and clinical applications of ADSCs under the influence of PBM.
光生物调节作用（PBM）通过光发射刺激细胞功能，最近在再生医学领域引起了广泛关注。本研究探讨了两种波长的PBM对人脂肪来源干细胞（ADSCs）的影响及其在体外分泌的外泌体的特征。从人脂肪组织中分离出ADSCs，并用650nm和810nm波长的PBM进行处理。使用MTT法、扫描电子显微镜（SEM）、BCA法测定外泌体蛋白浓度以及动态光散射（DLS）评估外泌体大小分布，对包括细胞活力、增殖和外泌体特征在内的治疗效果进行评估。结果表明，两种波长的PBM均显著提高了ADSCs的活力和增殖能力，MTT分析证实了这一点（650nm激光组p = 0.008，810nm激光组p = 0.007）。此外，外泌体分析显示，通过BCA和DLS测量，PBM处理组的外泌体蛋白浓度更高，平均大小约为86nm。我们得出结论，650nm和810nm波长的PBM分别显著增强了ADSCs的增殖和存活能力，并在体外改善了外泌体质量。该处理增加了外泌体蛋白浓度并改善了大小分布，反映出细胞功能增强。这些发现表明，PBM可能是一种有前景的刺激方法，可用于改善再生医学中基于ADSCs的治疗，特别是对于组织修复和慢性伤口愈合。需要进一步研究以探索PBM影响下ADSCs的分子机制和临床应用。
