Student Research Committee at Shahid Beheshti University of Medical Sciences (SBMU) in, Tehran, Iran.
Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences, Arabi Ave, Iran.
Lasers Med Sci. 2024 Mar 5;39(1):86. doi: 10.1007/s10103-024-04034-x.
In this preclinical investigation, we examined the effects of combining preconditioned diabetic adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation (PBM) on a model of infected ischemic delayed healing wound (injury), (IIDHWM) in rats with type I diabetes (TIDM). During the stages of wound healing, we examined multiple elements such as stereology, macrophage polarization, and the mRNA expression levels of stromal cell-derived factor (SDF)-1α, vascular endothelial growth factor (VEGF), hypoxia-induced factor 1α (HIF-1α), and basic fibroblast growth factor (bFGF) to evaluate proliferation and inflammation. The rats were grouped into: (1) control group; (2) diabetic-stem cells were transversed into the injury site; (3) diabetic-stem cells were transversed into the injury site then the injury site exposed to PBM; (4) diabetic stem cells were preconditioned with PBM and implanted into the wound; (5) diabetic stem cells were preconditioned with PBM and transferred into the injury site, then the injury site exposed additional PBM. While on both days 4, and 8, there were advanced histological consequences in groups 2-5 than in group 1, we found better results in groups 3-5 than in group 2 (p < 0.05). M1 macrophages in groups 2-5 were lower than in group 1, while groups 3-5 were reduced than in group 2 (p < 0.01). M2 macrophages in groups 2-5 were greater than in group 1, and groups 3-5 were greater than in group 2. (p ≤ 0.001). Groups 2-5 revealed greater expression levels of bFGF, VEGF, SDF- 1α, and HIF- 1α genes than in group 1 (p < 0.001). Overall group 5 had the best results for histology (p < 0.05), and macrophage polarization (p < 0.001). AD-MSC, PBM, and AD-MSC + PBM treatments all enhanced the proliferative stage of injury repairing in the IIDHWM in TIDM rats. While AD-MSC + PBM was well than the single use of AD-MSC or PBM, the best results were achieved with PBM preconditioned AD-MSC, plus additional PBM of the injury.
在这项临床前研究中，我们研究了预处理的糖尿病脂肪间充质干细胞（AD-MSCs）与光生物调节（PBM）联合应用于 I 型糖尿病（TIDM）大鼠感染性缺血延迟愈合创面（IIDHWM）模型的效果。在创面愈合的各个阶段，我们通过体视学、巨噬细胞极化以及基质细胞衍生因子（SDF）-1α、血管内皮生长因子（VEGF）、缺氧诱导因子 1α（HIF-1α）和碱性成纤维细胞生长因子（bFGF）的 mRNA 表达水平来评估增殖和炎症情况。将大鼠分为以下几组：（1）对照组；（2）糖尿病干细胞转染至损伤部位；（3）糖尿病干细胞转染至损伤部位后，损伤部位暴露于 PBM；（4）用 PBM 预处理糖尿病干细胞并植入创面；（5）用 PBM 预处理糖尿病干细胞并转移至损伤部位，然后损伤部位再接受额外的 PBM 照射。虽然在第 4 天和第 8 天，组 2-5 的组织学结果均优于组 1，但我们发现组 3-5 的结果优于组 2（p<0.05）。组 2-5 的 M1 巨噬细胞低于组 1，而组 3-5 的 M1 巨噬细胞低于组 2（p<0.01）。组 2-5 的 M2 巨噬细胞多于组 1，组 3-5 的 M2 巨噬细胞多于组 2（p≤0.001）。组 2-5 的 bFGF、VEGF、SDF-1α 和 HIF-1α 基因表达水平均高于组 1（p<0.001）。总体而言，组 5 的组织学结果（p<0.05）和巨噬细胞极化结果（p<0.001）最佳。AD-MSC、PBM 和 AD-MSC+PBM 治疗均增强了 TIDM 大鼠 IIDHWM 损伤修复的增殖期。虽然 AD-MSC+PBM 优于单独使用 AD-MSC 或 PBM，但使用 PBM 预处理 AD-MSC 并对损伤部位进行额外的 PBM 照射效果最佳。
