Dewhurst S, Sang J H
In Vitro. 1977 May;13(5):305-10. doi: 10.1007/BF02616176.
A saline extract was prepared from Drosophila eggs. When diluted to a concentration of 1% with Drosophila tissue culture medium, it did not support growth of cells from the Drosophila line D1 during the first few days of subculture as well as medium containing serum. When cells reached a stationary phase, however, the cell density in medium containing extract was greater than in medium containing serum. By altering the concentrations of the extract, and by adding bovine albumin, a medium was obtained in which D1 cells survived initial culturing, and which supported cell growth by day 4 as well as medium plus serum. The initial retardation of growth in medium containing egg extract might be due to the need of the cells to adapt to the new medium. At the present time four Drosophila cell lines have been maintained in this medium for more than 16 passages. Preliminary experiments with primary embryonic Drosophila cells indicate that medium containing 2% extract and bovine albumin retards the differentiation of these cells.
从果蝇卵中制备了一种盐提取物。当用果蝇组织培养基稀释至1%的浓度时，在继代培养的最初几天，它不像含血清的培养基那样支持果蝇D1系细胞的生长。然而，当细胞达到静止期时，含提取物的培养基中的细胞密度高于含血清的培养基。通过改变提取物的浓度，并添加牛血清白蛋白，获得了一种培养基，在其中D1细胞在初始培养中存活下来，并且到第4天时能像含血清的培养基一样支持细胞生长。含卵提取物的培养基中生长的最初延迟可能是由于细胞需要适应新培养基。目前，四个果蝇细胞系已在这种培养基中传代培养超过16次。对原代果蝇胚胎细胞的初步实验表明，含2%提取物和牛血清白蛋白的培养基会延迟这些细胞的分化。
