Steiss J E
Am J Vet Res. 1985 Oct;46(10):2141-4.
Vitamin E deficiency myopathy (white muscle disease) was induced in 14 suckling lambs (2 experiments; 7 lambs/experiment) by addition of cod liver oil to the diet. Disulfiram, an antioxidant, was administered orally once each day to 8 of the 14 lambs at 2 different doses. Serum creatine kinase (CK) activity was measured weekly for 5 weeks. Increased CK activity was evident in some lambs beginning at week 3. By week 4, serum CK was abnormally increased in 5 of the 6 nontreated lambs (ie, disulfiram not given) and in 4 of the 8 treated lambs. The combined disulfiram groups had significantly lower serum CK values during the study (P less than 0.05). Serum alpha-tocopherol, measured on samples from week 5 for lambs of experiment 1, was significantly higher in treated lambs (P less than 0.01). Microscopic examination of the vastus lateralis muscle indicated that the most severe lesions, consistent with nutritional myopathy, were seen in nontreated lambs. Therefore, disulfiram may have an antioxidant effect in lambs with vitamin E deficiency.
通过在饮食中添加鱼肝油，在14只哺乳羔羊（2个实验；每个实验7只羔羊）中诱发维生素E缺乏性肌病（白肌病）。抗氧化剂双硫仑以2种不同剂量每日一次口服给予14只羔羊中的8只。连续5周每周测量血清肌酸激酶（CK）活性。从第3周开始，一些羔羊的CK活性明显增加。到第4周时，6只未治疗的羔羊（即未给予双硫仑）中有5只以及8只治疗的羔羊中有4只血清CK异常升高。在研究期间，联合双硫仑组的血清CK值显著较低（P小于0.05）。对实验1中第5周羔羊样本测量的血清α-生育酚，治疗的羔羊显著更高（P小于0.01）。股外侧肌的显微镜检查表明，未治疗的羔羊出现了与营养性肌病一致的最严重病变。因此，双硫仑可能对维生素E缺乏的羔羊具有抗氧化作用。
