Mihas A A, Spenney J G
Int Arch Allergy Appl Immunol. 1977;54(3):255-61. doi: 10.1159/000231834.
Immunization of rabbits with a soluble human liver fraction incorporated into Freund's adjuvants produced antibodies that reacted with human liver extracts as well as extracts of other human organs. Absorption of the antihuman liver sera (AHLS) with lyophilized human serum, heart, and kidney abolished the reactions with other organs but did not remove the liver antibodies. The existence of at least two different liver antigens could be shown by gel diffusion precipitation experiments. One of these antigens could be removed by absorption of the antisera with chicken liver extracts. The AHLS did not crossreact with liver extracts of other species. Comparison of the AHLS with a mouse liver anti-serum described previously indicated that these antisera detected closely related but non-identical liver antigens.
用掺入弗氏佐剂的可溶性人肝组分免疫家兔，产生的抗体可与人肝提取物以及其他人体器官提取物发生反应。用冻干的人血清、心脏和肾脏吸收抗人肝血清（AHLS）后，与其他器官的反应消失，但肝脏抗体并未去除。凝胶扩散沉淀实验表明至少存在两种不同的肝脏抗原。其中一种抗原可通过用鸡肝提取物吸收抗血清而去除。AHLS与其他物种的肝脏提取物不发生交叉反应。将AHLS与先前描述的小鼠肝抗血清进行比较表明，这些抗血清检测到的是密切相关但不相同的肝脏抗原。
