Tinev Megi, Kristanc Luka, Gomišček Gregor, Božič Bojan
Faculty of Medicine, Institute of Biophysics, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia.
Faculty of Health Sciences, University of Novo mesto, Na Loko 2, 8000, Novo mesto, Slovenia.
J Membr Biol. 2025 Jul 7. doi: 10.1007/s00232-025-00354-3.
The passage of nystatin through the ergosterol-containing phospholipid bilayer was studied on monovesicular and multivesicular giant unilamellar vesicles (GUVs and MVVs). Phase-contrast optical microscopy was used to examine vesicles composed of a palmitoyl-oleoyl-phosphatidyl-choline (POPC) bilayer with either 15 or 45 mol% ergosterol. Three types of vesicles were analyzed: (i) GUVs, (ii) outer vesicles (outGUVs) of MVVs, and (iii) inner vesicles (inGUVs) of MVVs. The times of their ruptures were determined after their exposure to nystatin at concentrations of 250 and 500 M. At both concentrations, the times that the inGUVs spent in the nystatin solution after the rupture of the corresponding outGUVs were significantly shorter than the rupture times of individual GUVs of the similar size. These differences in rupture times demonstrate that the ergosterol-containing POPC membrane is permeable to nystatin.
在单泡和多泡巨型单层囊泡（GUV和MVV）上研究了制霉菌素通过含麦角固醇的磷脂双层的过程。利用相差光学显微镜检查由含有15%或45%摩尔麦角固醇的棕榈酰油酰磷脂酰胆碱（POPC）双层组成的囊泡。分析了三种类型的囊泡：（i）GUV，（ii）MVV的外层囊泡（outGUV），以及（iii）MVV的内层囊泡（inGUV）。在它们暴露于浓度为250和500 μM的制霉菌素后，测定它们破裂的时间。在这两种浓度下，相应的outGUV破裂后inGUV在制霉菌素溶液中停留的时间明显短于类似大小的单个GUV的破裂时间。这些破裂时间的差异表明含麦角固醇的POPC膜对制霉菌素是可渗透的。
