Bartoshevich Iu E, Novak M I, Iudina O D, Sazykin Iu O, Beliaevskaia I V
Antibiot Med Biotekhnol. 1985 Jun;30(6):410-4.
Dependence of the content of intracellular methionine and its distribution between the fraction of the high-molecular intracellular compounds and the cytoplasmic amino acid pool in the cephalosporin C-producing organism A. chrysogenum on the content of the carbohydrate source in the medium was studied. In the presence of both glucose and sucrose accumulation of methionine in the cells was observed prior to the antibiotic production. With the use of 35S- or 2(14)C-methionine it was shown that glucose had no repressing effect on methionine transport by the cells of different age (24 and 72 hours). In the presence of glucose the higher levels of 35S- or 2(14)C-methionine were detected in the fraction of the low-molecular compounds of the cells within the first 36 hours of the culture growth. Apparently, the intracellular methionine was used to a larger extent for protein synthesis rather than for construction of the antibiotic molecule.
研究了头孢菌素C产生菌产黄青霉中细胞内蛋氨酸含量及其在高分子量细胞内化合物部分和细胞质氨基酸库之间的分布对培养基中碳水化合物源含量的依赖性。在葡萄糖和蔗糖同时存在的情况下，在抗生素产生之前观察到细胞中蛋氨酸的积累。使用35S-或2(14)C-蛋氨酸表明，葡萄糖对不同年龄（24小时和72小时）细胞的蛋氨酸转运没有抑制作用。在葡萄糖存在的情况下，在培养生长的前36小时内，细胞低分子化合物部分中检测到较高水平的35S-或2(14)C-蛋氨酸。显然，细胞内蛋氨酸在很大程度上用于蛋白质合成，而不是用于抗生素分子的构建。
