[Effect of carbohydrate catabolic repression on the accumulation and intracellular distribution of methionine in Acremonium chrysogenum--the producer of cephalosporin C].

Dependence of the content of intracellular methionine and its distribution between the fraction of the high-molecular intracellular compounds and the cytoplasmic amino acid pool in the cephalosporin C-producing organism A. chrysogenum on the content of the carbohydrate source in the medium was studied. In the presence of both glucose and sucrose accumulation of methionine in the cells was observed prior to the antibiotic production. With the use of 35S- or 2(14)C-methionine it was shown that glucose had no repressing effect on methionine transport by the cells of different age (24 and 72 hours). In the presence of glucose the higher levels of 35S- or 2(14)C-methionine were detected in the fraction of the low-molecular compounds of the cells within the first 36 hours of the culture growth. Apparently, the intracellular methionine was used to a larger extent for protein synthesis rather than for construction of the antibiotic molecule.