Ippolito Michael, Maddox Charlie, Inam Amal, Wimmer Mathieu E, Ward Sara Jane
Center for Substance Abuse Research, Department of Neural Sciences, Temple University;
Center for Substance Abuse Research, Department of Neural Sciences, Temple University.
J Vis Exp. 2025 Jun 20(220). doi: 10.3791/68079.
This study demonstrates the utility of a rat model of chemotherapy-induced peripheral neuropathy (CIPN) to assess the ability of the non-psychoactive cannabinoid cannabidiol (CBD) to modulate the development of this syndrome in vivo. The method utilizes the chemotherapeutic agent paclitaxel to generate an allodynic phenotype in the animals. This study describes how to handle and solubilize CBD, administer the chemotherapeutic agent, assess mechanical and cold sensitivity, and apply high-speed videography to measure nocifensive behavior in animals. Using the procedures outlined, the data support that CBD prevents the allodynic phenotype from developing in the treated animals. No difference was observed in the CBD-treated animals from day 0 (pre-paclitaxel baseline) to day 7 (post-sensitization) in mechanical or thermal sensitivity, while the vehicle-treated animals became significantly more sensitive. This response to treatment is durable up to the latest time point where data were collected (7 weeks). The addition of high-speed videography allows for a more granular and unbiased assessment of this behavioral phenotype (e.g., classification of analgesia and anti-allodynia). This demonstrates both the utility of this model for cannabinoid drug characterization and the potential role of CBD in mitigating neuropathic pain.
本研究证明了化疗诱导的周围神经病变（CIPN）大鼠模型在评估非精神活性大麻素大麻二酚（CBD）在体内调节该综合征发展能力方面的实用性。该方法利用化疗药物紫杉醇在动物中产生痛觉过敏表型。本研究描述了如何处理和溶解CBD、施用化疗药物、评估机械和冷敏感性，以及应用高速摄像来测量动物的防御性疼痛行为。使用所述程序，数据支持CBD可防止在接受治疗的动物中出现痛觉过敏表型。在接受CBD治疗的动物中，从第0天（紫杉醇治疗前基线）到第7天（致敏后），在机械或热敏感性方面未观察到差异，而接受赋形剂治疗的动物变得明显更敏感。这种对治疗的反应在收集数据的最晚时间点（7周）之前都是持久的。高速摄像的加入使得对这种行为表型的评估更加细致且无偏差（例如，镇痛和抗痛觉过敏的分类）。这既证明了该模型在大麻素药物表征方面的实用性，也证明了CBD在减轻神经性疼痛方面的潜在作用。
