Menasché P, Pinard E, Desroches A M, Seylaz J, Laget P, Geyer R P, Piwnica A
Ann Thorac Surg. 1985 Nov;40(5):494-7. doi: 10.1016/s0003-4975(10)60106-3.
This study was designed to assess whether an oxygenated fluorocarbon solution could reduce ischemic brain damage related to arterial air embolism. Air embolism was produced by injecting air bubbles into the carotid artery of barbiturate-anesthetized rats breathing 100% oxygen. Results were assessed on electrocorticogram. In an additional set of experiments, mass spectrometry was used to provide continuous monitoring of intracerebral tissue oxygen (PO2) and carbon dioxide (PCO2) tensions and intermittent measurement of cerebral blood flow (CBF). Fluorocarbon or saline solution (containing the emulsifying agent of fluorocarbons) was given intravenously after the initial air embolism (0.2 ml), and injections of air (0.1 ml) were repeated thereafter every five minutes. The maximal amount of air required to achieve complete and irreversible flattening of the electrocorticogram was 1.60 +/- 0.06 ml (mean +/- standard error of the mean) in the saline-treated rats and 5.20 +/- 0.44 ml in the fluorocarbon-treated group (p less than 10(-7)). In the second experiment, air embolism caused CBF to rise in both groups, the average percent of increase being higher in treated (41.6%) than in control animals (38.3%) (p less than 0.02). However, in the control group, the increase in CBF did not prevent intracerebral tissue PO2 from decreasing by 7.4 +/- 7.0% over the same period; conversely, in the fluorocarbon group, PO2 levels fell by only 2.5 +/- 3.7% (p less than 0.001 versus controls), but this time-averaged percentage was calculated over a longer period of cumulative ischemia because of the greater number of air emboli tolerated by treated animals.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在评估充氧氟碳溶液是否能减轻与动脉空气栓塞相关的缺血性脑损伤。通过向呼吸100%氧气的巴比妥类麻醉大鼠的颈动脉注射气泡来制造空气栓塞。根据脑电图评估结果。在另一组实验中，使用质谱法持续监测脑组织氧（PO2）和二氧化碳（PCO2）张力，并间歇性测量脑血流量（CBF）。在首次空气栓塞（0.2 ml）后静脉注射氟碳溶液或盐溶液（含氟碳乳化剂），此后每五分钟重复注射空气（0.1 ml）。在盐水处理组大鼠中，使脑电图完全不可逆平坦所需的最大空气量为1.60±0.06 ml（平均值±平均值的标准误差），在氟碳处理组中为5.20±0.44 ml（p＜10⁻⁷）。在第二个实验中，空气栓塞使两组的脑血流量均升高，治疗组的平均升高百分比（41.6%）高于对照组动物（38.3%）（p＜0.02）。然而，在对照组中，脑血流量的增加并未阻止同期脑组织PO2下降7.4±7.0%；相反，在氟碳组中，PO2水平仅下降2.5±3.7%（与对照组相比p＜0.001），但由于治疗组动物耐受的空气栓子数量更多，这个时间平均百分比是在更长的累积缺血期计算得出的。（摘要截断于250字）
