Giorgi F
Histochemistry. 1977 May 20;52(2):105-17. doi: 10.1007/BF00492287.
Ovarian follicle cells of Drosophila melanogaster have been studied by ultrastructural and autoradiographic analyses. During their migration through the germarium, follicle cells undergo several structural changes and, of these, the most conspicuous one occurs at the level of the nucleolus. By the time the first ovarian chamber is formed, follicle cells have formed a layer of uniform thickness all around a cluster or nurse cells and the oocyte. Following the initiation of vitellogenesis, the follicle cells overlaying the oocyte become columnar while those over the nurse cells become very thin. During stages 9-10, the columnar follicle cells are involved in the formation of the vitelline membrane, while from stages 11 to 13 these cells produce the endochorion. An EM autoradiographic analysis has shown that the rate of 3H-uridine incorporation in follicle cells nuclei is low in previtellogenic chambers, while it becomes very high in nuclei of stage 9-10 chambers. After short exposure to uridine, silver grains are located predominantly over nucleoli. Evidence from incorporation studies with 3H-lysine indicates that the columnar follicle cells and the region of the various egg coverings are highly labelled within an hour of incubation in the tracer. The observations confirm that columnar follicle cells are the only cells in the chamber involved in the formation of materials which make up the egg coverings.
已通过超微结构和放射自显影分析对黑腹果蝇的卵巢卵泡细胞进行了研究。在卵泡细胞穿过生殖腺的过程中，它们会经历几种结构变化，其中最明显的变化发生在核仁水平。当第一个卵巢腔形成时，卵泡细胞已在一群滋养细胞和卵母细胞周围形成了一层厚度均匀的细胞层。在卵黄发生开始后，覆盖卵母细胞的卵泡细胞变为柱状，而覆盖滋养细胞的卵泡细胞则变得非常薄。在第9 - 10阶段，柱状卵泡细胞参与卵黄膜的形成，而从第11阶段到第13阶段，这些细胞产生内 chorion（此处原文可能有误，推测为卵壳内膜endochorion）。电子显微镜放射自显影分析表明，在卵黄发生前的卵巢腔中，卵泡细胞核中3H - 尿苷的掺入率较低，而在第9 - 10阶段卵巢腔的细胞核中则变得非常高。在短时间暴露于尿苷后，银颗粒主要位于核仁上。用3H - 赖氨酸掺入研究的证据表明，在示踪剂中孵育一小时内，柱状卵泡细胞和各种卵壳区域都被高度标记。这些观察结果证实，柱状卵泡细胞是卵巢腔中唯一参与构成卵壳物质形成的细胞。
