Ghosh S K, Majumder S, Mukhopadhyay N K, Bose S K
Biochem J. 1985 Sep 15;230(3):785-9. doi: 10.1042/bj2300785.
The enzyme fraction A, a constituent enzyme of the three-fraction enzyme mycobacillin synthetase, independently and sequentially activated five amino acids starting from L-proline, producing the pentapeptide Pro(Asp1,Glu1,Tyr1)Asp. The fractions B and C were unable to function independently. However, the fraction B synthesized the nonapeptide Pro(Asp3,Glu1,Tyr2,Ser1)Leu, sequentially activating the pentapeptide and next four amino acids, whereas the fraction C synthesized mycobacillin by the sequential activation of the nonapeptide and the remaining four amino acids. The pH optima of the above enzymes are almost identical (pH 7.8), but their Km values are a little different.
酶组分A是三分组酶分枝杆菌素合成酶的一种组成酶，它从L-脯氨酸开始独立且依次激活5种氨基酸，生成五肽Pro(Asp1,Glu1,Tyr1)Asp。组分B和C不能独立发挥作用。然而，组分B合成了九肽Pro(Asp3,Glu1,Tyr2,Ser1)Leu，依次激活五肽和接下来的4种氨基酸，而组分C通过依次激活九肽和其余4种氨基酸合成了分枝杆菌素。上述酶的最适pH几乎相同（pH 7.8），但它们的米氏常数略有不同。
