利用组蛋白H1的N-溴代琥珀酰亚胺切割片段检测蛋白激酶C
Assay of protein kinase C with an N-bromosuccinimide-cleavage fragment of histone H1.
作者信息
Glynn B, Colliton J, McDermott J, Witters L A
出版信息
Biochem J. 1985 Oct 15;231(2):489-92. doi: 10.1042/bj2310489.
Abstract
N-Bromosuccinimide cleavage of a lysine-rich histone fraction (histone III-S) yields a peptide substrate, purified by reverse-phase h.p.l.c., for the Ca2+ + phospholipid-dependent protein kinase (protein kinase C). This substrate displays no reactivity with the cyclic AMP-dependent protein kinase, and may prove useful for the detection of protein kinase C activity in crude tissue extracts.
摘要
用N-溴代琥珀酰亚胺裂解富含赖氨酸的组蛋白组分（组蛋白III-S）可产生一种肽底物，该底物通过反相高效液相色谱法纯化，用于检测Ca2+ + 磷脂依赖性蛋白激酶（蛋白激酶C）。这种底物与环磷酸腺苷依赖性蛋白激酶没有反应性，可能对检测粗组织提取物中的蛋白激酶C活性有用。
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