Stern M D, Hoover W H, Summers R G, Rittenburg J H
J Dairy Sci. 1977 Jun;60(6):902-10. doi: 10.3168/jds.S0022-0302(77)83961-1.
Thin sections of rumen ciliated protozoa of the subclass Spirotrichia were studied by electron microscopy to elucidate their ultrastructure. To prevent retraction of their adoral cilia, menthol crystals were used to relax the retrociliary region. These protozoa had a distinct ectoplasm and endoplasm with the macro- and micronuclei located in the ectoplasm. At the surface of the entodiniomorph body was a highly differentiated cortical zone of four layers. Ribosomes were abundant throughout the cytoplasm, suggesting a substantial potential for protein synthesis. These protozoa appeared to engulf bacteria into large vacuoles, and subsequently the bacteria were taken into the endoplasm in vesicles containing only one bacterium each. The bacteria were digested partially, and only in isolated cases were the bacterial cell walls still intact.
通过电子显微镜研究了螺旋毛亚纲瘤胃纤毛虫的薄片，以阐明其超微结构。为防止口前纤毛回缩，使用薄荷醇晶体使纤毛后区域松弛。这些原生动物有明显的外质和内质，大核和小核位于外质中。内毛形体表面是一个高度分化的四层皮质区。核糖体在整个细胞质中丰富，表明有大量蛋白质合成的潜力。这些原生动物似乎将细菌吞噬到大型液泡中，随后细菌被摄入内质中，每个囊泡中只含有一个细菌。细菌被部分消化，仅在个别情况下细菌细胞壁仍保持完整。
