Samukov V V, Kalashnikov V V, Ofitserov V I, Shval'e A F
Bioorg Khim. 1985 Aug;11(8):1037-47.
Peptides corresponding to sequences 122-133, 136-147, and 154-164 of the heavy chain of hemagglutinin of the A/Aichi/2/68 (H3N2) influenza virus have been synthesized by stepwise elongation of the peptide chain with Boc-amino acid activated esters or by condensation of peptide blocks by DCC/HOBt-method. A coloured C-protecting group, 2-[4-(phenylazo)-benzylsulfonyl]ethyl (PSE), was used, which is convenient in purification of synthetic peptides. After removal of terminal N-and C-protecting groups the side-protecting residues were cleaved off with 1 M trifluoromethanesulfonic acid in trifluoroacetic acid containing 10% thioanisole. Crude products were purified by preparative reversed-phase liquid chromatography. Synthesized peptides were conjugated with BSA.
通过使用Boc-氨基酸活化酯逐步延长肽链或通过DCC/HOBt方法缩合肽段，合成了与A/爱知/2/68（H3N2）流感病毒血凝素重链的122-133、136-147和154-164序列相对应的肽。使用了一种有色的C保护基团，即2-[4-（苯偶氮）-苄基磺酰基]乙基（PSE），这便于合成肽的纯化。去除末端N-和C-保护基团后，用含10%硫代苯甲醚的三氟乙酸中的1 M三氟甲磺酸裂解侧链保护残基。粗产物通过制备型反相液相色谱法纯化。合成的肽与牛血清白蛋白（BSA）偶联。
