Seys Sven F, Gherasim Alina, Odul Florian, Dietsch Frank, Diamant Zuzana, Gevaert Philippe, Hellings Peter W, Loeckx Dirk, Gorris Senne, de Blay Frédéric
Hippo Dx, Aarschot, Belgium.
Centre de Recherche Clinique ALYATEC, Strasbourg, France.
Allergy. 2025 Jul 9. doi: 10.1111/all.16645.
A novel device, Skin Prick Automated Test (SPAT), previously showed reduced variability and more consistent test results compared to conventional skin prick test (SPT) to identify allergic sensitisation. This study aimed to clinically validate the adjusted SPAT cut-off in patients with confirmed birch or house dust mite (HDM) allergy.
Seventy-five adults were included: 25 non-allergic subjects (confirmed by lack of allergy history and negative SPT), 25 birch and 25 HDM allergic rhinitis patients (both confirmed by positive SPT and nasal allergen challenge [NAC]). All subjects received a conventional SPT and an automated SPT for B ver, D pter, D far and control solutions.
A cut-off of 4.2 and 4.1 mm, respectively, resulted in the highest accuracy to detect birch or HDM allergy using SPAT. Referring to previous study results suggesting a reliable cut-off value of 4.5 mm, it was decided to maintain 4.5 mm as SPAT cut-off indicating allergic sensitisation. Accuracy did not significantly differ between SPAT (96% using 4.5 mm) and conventional SPT (98% using 3.0 mm) to detect HDM allergy or to detect birch pollen allergy (100% for SPAT and SPT). SPAT wheal measurements performed through a ruler on the forearm or through digital measurement on a composite image did not significantly differ for any of the patient groups analysed.
SPAT showed an equivalent accuracy to detect birch pollen or HDM allergy compared to conventional SPT, using the adjusted 4.5 mm SPAT cut-off in patients with confirmed allergic rhinitis. The SPAT web viewer can be used easily and effectively for digital wheal measurement on a composite image.
一种新型设备，即皮肤点刺自动测试（SPAT），与传统皮肤点刺试验（SPT）相比，先前显示其变异性降低且测试结果更一致，用于识别过敏致敏情况。本研究旨在对确诊为桦树或屋尘螨（HDM）过敏的患者中调整后的SPAT临界值进行临床验证。
纳入75名成年人：25名非过敏受试者（通过缺乏过敏史和阴性SPT确诊），25名桦树过敏和25名HDM过敏性鼻炎患者（均通过阳性SPT和鼻过敏原激发试验[NAC]确诊）。所有受试者均接受针对桦树花粉、粉尘螨、屋尘螨和对照溶液的传统SPT和自动SPT。
分别以4.2毫米和4.1毫米的临界值，使用SPAT检测桦树或HDM过敏的准确性最高。参考先前研究结果表明可靠的临界值为4.5毫米，决定将4.5毫米作为表明过敏致敏的SPAT临界值。在检测HDM过敏或桦树花粉过敏方面，SPAT（使用4.5毫米时为96%）和传统SPT（使用3.0毫米时为98%）的准确性没有显著差异（SPAT和SPT检测桦树花粉过敏的准确率均为100%）。对于所分析的任何患者组，通过在前臂上用尺子测量或在合成图像上进行数字测量得到的SPAT风团测量结果没有显著差异。
在确诊过敏性鼻炎的患者中，使用调整后的4.5毫米SPAT临界值时，SPAT在检测桦树花粉或HDM过敏方面显示出与传统SPT相当的准确性。SPAT网络查看器可轻松有效地用于在合成图像上进行数字风团测量。
